| 甘氨酸-N-甲基转移酶对神经干细胞分化的影响 |
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| 引用本文： | 李海龙,郭志华,夏丽萍,文铁桥.甘氨酸-N-甲基转移酶对神经干细胞分化的影响[J].科技通报,2005,21(3):262-265,279. |
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| 作者姓名： | 李海龙 郭志华 夏丽萍 文铁桥 |
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| 作者单位： | 上海大学,生命科学学院,上海,200436 |
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| 基金项目： | 国家973计划资助(G1999054000)。 |
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| 摘 要： | 本文主要是研究甘氨酸-N-甲基转移酶(Gnmt)抑制后对神经干细胞分化的影响。从14dSD孕鼠的胚胎纹状体分离培养神经干细胞，培养两周后，将神经干细胞接种到24孔板，加入甘氨酸-N-甲基转移酶的产物SAH对该酶进行抑制，观察其抑制后对神经干细胞分化的影响。采用RT-PCR分析Gnmt抑制后mRNA的表达。结果表明：甘氨酸-N-甲基转移酶抑制可以诱导神经干细胞的分化。
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| 关 键 词： | 神经分子生物学 神经干细胞 细胞分化 Gnmt 基因调控 |
| 文章编号： | 1001-7119(2005)03-0262-04 |
The Effect of Glycine-N-Methyltransferase on the Neural Stem Cells |
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| LI Hai-long,GUO Zhi-Hua,XIA Li-ping,WEN Tie-qiao.The Effect of Glycine-N-Methyltransferase on the Neural Stem Cells[J].Bulletin of Science and Technology,2005,21(3):262-265,279. |
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| Authors: | LI Hai-long GUO Zhi-Hua XIA Li-ping WEN Tie-qiao |
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| Abstract: | The paper studied the effect of glycine-N-methyltransferase(Gnmt) on the neural stem cells. The neural stem cells from the striatum corpora of rat's embryo were cultrued in 24 well for 14 days. Inhibitor, SAH, a sort of substrate of Gnmt was added into the culture medium, and the effect of the Gnmt expression in the level of nRNA on NSCs differentiation was examined by RT-PCR. The down regulation of Gnmt. Could induce the differentiation of NSCs. |
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| Keywords: | neural molecular biology neural stem cell cell differentation Gnmt gene regulation |
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