| SIRTUIN1介导的NIH3T3成纤维细胞昼夜节律时钟基因表达抑制的光生物调节作用 |
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| 引用本文： | 吴德峰,刘承宜,朱玲.SIRTUIN1介导的NIH3T3成纤维细胞昼夜节律时钟基因表达抑制的光生物调节作用[J].体育科学,2011,31(2). |
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| 作者姓名： | 吴德峰 刘承宜 朱玲 |
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| 作者单位： | 华南师范大学,广东,广州,510006 |
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| 基金项目： | 国家自然科学基金(60878061) |
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| 摘 要： | 背景和目的:昼夜节律是影响运动成绩的一个重要因素。研究发现,介导运动应激的肿瘤坏死因子(tumor necrosis factor,TNF)能够抑制昼夜节律时钟基因的表达。研究了低强度810nm激光(low intensity 810 nm laserir radiation,LIL)对TNF抑制效应的调节作用。材料和方法:50%马血清休克处理NIH3T3成纤维细胞2h,同步化昼夜节律时钟基因的表达后,加入10ng/mL TNF-alpha抑制它的表达,与此同时给予20min10mW/cm2的LIL照射。36h内,每隔6h检测细胞中时钟基因Clock、Bmal1、Per2、Dbp和烟酰胺腺嘌呤二核苷酸辅酶(nicotinamide adenine dinucleotide,NAD+)依赖的组蛋白去乙酰化酶1(sirtuin1,Sirt1)的mRNA的表达及胞内NAD+和其还原形式NADH的比值NAD+/NADH。结果:TNF-alpha分别在第12h和第18h抑制了Clock(P<0.05)、第18h抑制了Bmall(P<0.05)和Dbp(P<0.005)、第18h和第30h抑制了Per2(P<0.05)及...
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| 关 键 词： | 昼夜节律时钟基因 肿瘤坏死因子 光生物调节作用 |
Sirtuin1-mediated Photobiomodulation on TNF-alpha Inhibited Expression of Circadian Clock Genes in Cultured NIH3T3 Fibroblasts |
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| WU De-feng,LIU Cheng-yi,ZHU Ling.Sirtuin1-mediated Photobiomodulation on TNF-alpha Inhibited Expression of Circadian Clock Genes in Cultured NIH3T3 Fibroblasts[J].China Sport Science,2011,31(2). |
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| Authors: | WU De-feng LIU Cheng-yi ZHU Ling |
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| Institution: | WU De-feng,LIU Cheng-yi,ZHU Ling South China Normal University,Guangzhou 510006,China |
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| Abstract: | Background and Objective:Circadian rhythm is one of the important factors on athletic performance.Tumor necrosis factor(TNF),which mediates sport stress,has been shown to inhibit the expression of circadian Clock genes.The modulation of low intensity 810 nm laser irradiation(LIL) on the inhibition was studied in this paper.Study design/materials and method:The expression of the circadian Clock genes in NIH3T3 fibroblasts was synchronized by 50% horse serum shock for 2h,inhibited with TNF-alpha at 10ng/mL,an... |
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| Keywords: | circadian Clock genes tumor necrosis factor(TNF) photobiomodultion |
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