| 高等植物不同类型细胞的分离技术 |
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| 引用本文： | 丁锐,朱延姝,崔震海,马蓉,胡逢雪,于丽姣,张立军.高等植物不同类型细胞的分离技术[J].科技通报,2012(1):80-85. |
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| 作者姓名： | 丁锐 朱延姝 崔震海 马蓉 胡逢雪 于丽姣 张立军 |
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| 作者单位： | 沈阳农业大学生物技术学院 |
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| 基金项目： | 国家自然科学基金项目(30870190);教育部博士点基金项目(200801570005),教育部博士点基金项目(20102103120001);辽宁省教育厅科研基金项目(20060784),辽宁省教育厅高校科研计划项目(2008S211);沈阳农业大学青年基金项目(2007)资助,沈阳农业大学博士后流动站资助 |
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| 摘 要： | 高等植物由许多形态和功能不同的细胞组成,各类细胞在植物体内行使特定的功能,同时,细胞间又存在着结构和功能上的密切联系。要想获得特定类型细胞的准确信息,必须首先将其从杂合的细胞群中分离出来。高等植物细胞分离技术主要有密度梯度离心法、流式细胞仪法、激光捕获显微切割技术和人工显微分离技术等。本文综合介绍了这些方法的基本原理、操作流程及优缺点,并介绍了近年来高等植物细胞分离方面的研究进展。
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| 关 键 词： | 高等植物 细胞分离 原生质体分离 密度梯度离心 流式细胞仪 激光捕获显微切割 |
Technology of Isolation of Different Types of Cells from Higher Plants |
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| DING Rui,ZHU Yanshu,CUI Zhenhai,MA Rong,HU Fengxue,YU Lijiao,ZHANG Lijun.Technology of Isolation of Different Types of Cells from Higher Plants[J].Bulletin of Science and Technology,2012(1):80-85. |
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| Authors: | DING Rui ZHU Yanshu CUI Zhenhai MA Rong HU Fengxue YU Lijiao ZHANG Lijun |
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| Institution: | (College of Life Science and Technology,Shenyang Agricultural University,Shenyang 110161,China) |
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| Abstract: | Higher plants are composed of many kinds of cells with different functions.Each kind of cells have their specific functions in plants,meanwhile,there are close relations between their structures and functions.In order to obtain accurate information of certain type of cells,at first,we must separate them from the mixed cells.The methods of separating higher plant cells could be divided into four kinds:density gradient centrifugation,flow cytometry,laser capture microdissection and microseparation.In this paper,we review these methods’ basic principles,operation procedures and their advantages and disadvantages;we also present the progress of their applications in higher plant cells separation in recent years. |
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| Keywords: | higher plant cell separation protoplast isolation density gradient centrifugation flow cytometry laser capture microdissection |
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