| Science Letters： A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana |
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| 作者姓名： | Chen B Jiang JH Zhou XP |
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| 作者单位： | [1]State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China [2]College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China |
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| 基金项目： | Project supported by the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China （No. 705025） and the National Natural Science Foundation of China （No. 30530520） |
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| 摘 要： | The AtTOM1 gene ofArabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus （TMV） in A. thaliana. In this study, we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1. Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N. tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities, respectively. Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N. benthamiana. The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants.
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| 关 键 词： | 本氏烟 烟草花叶病毒 NbTOM1 复制 病毒诱发基因沉默 |
| 收稿时间： | 2006-09-17 |
| 修稿时间： | 2006-11-18 |
A <Emphasis Type="Italic">TOM1</Emphasis> homologue is required for multiplication of Tobacco mosaic virus in <Emphasis Type="Italic">Nicotiana benthamiana</Emphasis> |
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| Chen B,Jiang JH,Zhou XP.A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana[J].Journal of Zhejiang University Science,2007,8(4):256-259. |
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| Authors: | Chen Bing Jiang Jin-hua Zhou Xue-ping |
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| Institution: | (1) State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, China;(2) College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China |
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| Abstract: | The AtTOM1 gene of Arabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus (TMV) in A. thaliana. In this study, we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1. Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N. tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities, respectively. Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N. benthamiana. The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants.
Project supported by the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of
China (No. 705025) and the National Natural Science Foundation of China (No. 30530520) |
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| Keywords: | Tobacco mosaic virus NbTOM1 Virus induced gene silencing Nicotiana benthamiana |
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