Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein |
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| Authors: | Email author" target="_blank">Luo?Ben-yanEmail author Xu?Zeng-bin Chen?Zhi Chen?Feng Tang?Min |
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| Institution: | (1) Department of Neurology First Affiliated Hospital Medical College, Zhejiang University, 310003 Hangzhou, China;(2) Department of Radiology First Affiliated Hospital, Medical College, Zhejiang University, 310003 Hangzhou, China;(3) Institute of Infectious Diseases First Affiliated Hospital, Medical College, Zhejiang University, 310003 Hangzhou, China |
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| Abstract: | Objective: To construct a PC 12 cell strain with neuronal differentiation, and observe the apoptosis and proliferation activity
effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmic growth phase were subcultured
for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF and cultured for 9 days. 2) Neuronal
differentiation of PC 12 cells in logarithmic growth phase were divided into four groups: control group (0), experimental
group (1), experimental group (2) and experimental group (3). The concentrations of Aβ in the four groups were 0 μmol/L, 1.25
μmol/Land 5 μmol/L, respectively. The cells were harvested at 24, 48 and 72 h later and stained with Annexin V-FITC/PI after
centrifugation and washing. Then flow cytometry was conducted to examine the apoptosis percentage. 3) NGF-induced PC12 cells
were selected and Aβ with different concentrations was added. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5
μmol/L and 5 μmol/L, respectively. After the cells were incubated in an atmosphere of 5% CO2 at 37 °C in an incubator for 72 h, the OD values were examined. Results: 1) Neuronal differentiated PC12 cell lines were
successfully established. 2) Flow cytometric examination indicated that Aβ (1.25, 2.5, and 5.0 μmol/L) could effectively induce
apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h time points. 3) Aβ (0–5.00 μmol/L) had no obvious effect
on proliferation or restraining of the neuronal differentiation of the PC 12 cells after a 72 h interacting process. Conclusion:
This investigation revealed successful neuronal differentiation of the PC 12 cell strain. The induction of apoptosis of the
neurocytes by various concentrations of Aβ was observed and the influence of Aβ on induced proliferation of PC 12 cells by
Rat-β-NGF was revealed. This study may provide basis for future research on the molecular cure of AD and interdiction of AD
evolution.
Project (No. 2003B054) supported by the Public Health Bureau of Zhejiang Province, China |
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| Keywords: | Alzheimer's Disease Amyloid beta-Protein Nneurocytes Apoptosis |
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