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模拟酸雨对小麦幼苗SOD和CAT活性的影响
引用本文:唐正义,杨春萍,王丹,郑玉.模拟酸雨对小麦幼苗SOD和CAT活性的影响[J].内江师范学院学报,2014(4):30-32.
作者姓名:唐正义  杨春萍  王丹  郑玉
作者单位:内江师范学院生命科学学院,四川内江641199
基金项目:四川省教育厅重点项目(07ZC032);内江师范学院大学生科研项目(12NSD-41)
摘    要:分别用pH为2.0、3.0、4.0、5.6模拟酸雨对小麦幼苗进行急性毒理实验,用紫外分光光度法检测幼苗体内过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性.结果表明:pH在3.0左右时,小麦幼苗的CAT和SOD活性最高;在不同酸度的模拟酸雨的胁迫下,CAT和SOD活性的剂量效应关系曲线为单峰曲线,其峰值可以认为是小麦幼苗对酸雨中毒反应的阈值.结论:不同酸度的模拟酸雨对小麦体幼苗CAT和SOD酶活性有诱导作用.

关 键 词:模拟酸雨  小麦  过氧化氢酶  超氧化物歧化酶

Effects of Simulated Acid Rain on Wheat Seedling SOD and CAT Activity
TANG Zheng-yi,YANG Chun-ping,WANG Dan,ZHENG Yu.Effects of Simulated Acid Rain on Wheat Seedling SOD and CAT Activity[J].Journal of Neijiang Teachers College,2014(4):30-32.
Authors:TANG Zheng-yi  YANG Chun-ping  WANG Dan  ZHENG Yu
Institution:(College of Life Science, Neijiang Normal University, Neijiang, Sichuan 641199, China)
Abstract:Using simulated acid rains of pH values at 2.0, 3.0, 4.0 and 5.6, acute toxicity experiments were conducted on wheat seedlings to investigate the seedlings catalase spectrophotometry (CAT) and superoxide dismutase (SOD) activity by means of UV detection. The results showed that: CAT and SOD had the highest activity when the pH value was controlled at about 3 in wheat seedlings; under the stress of simulated acid rain with different acidity conditions, the dose-effect relationship curve of cAT and SOD activity takes aunimodal curve, and the peak value can be considered the threshold value of the toxic re- action of wheat seedlings exposed to acid rain. Conclusion: simulated acid rains of different acidity have different CAT and SOD enzyme induction on wheat seedlings.
Keywords:simulated acid rain  wheat  peroxidase  superoxide dismutase
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