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Antioxidant activity of alcohol aqueous extracts of <Emphasis Type="Italic">Crypthecodinium cohnii</Emphasis> and <Emphasis Type="Italic">Schizochytrium</Emphasis> sp.
Authors:Jin-hui Yu  Jie Sun  Fei Bian  Gao Chen  Yan Zhang  Yu-ping Bi  Ying-jie Wu
Institution:1.Institute of Genome Engineered Animal Models for Human Disease,Dalian Medical University,Dalian,China;2.Shandong Center of Crop Germplasm Resources,Jinan,China;3.School of Life Sciences,Shandong University,Jinan,China;4.College of Integrative Medicine,Dalian Medical University,Dalian,China;5.Biotechnology Research Center,Shandong Academy of Agricultural Sciences,Jinan,China
Abstract:Crypthecodinium cohnii (dinoflagellate) and Schizochytrium sp. (thraustochytrid) are the main sources for docosahexaenoic acid (DHA). The present study aimed to evaluate the antioxidant activity of petroleum ether, ethyl acetate, n-butanol, and water fractions of alcohol aqueous extracts of these two microalgae and to provide a theoretical basis for comprehensive utilization. The antioxidant activity was determined by total antioxidant capacity (TAC) determination, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, ferrous ion-chelating ability (FICA) assay, and reducing power (RP) assay. The total phenolic content (TPC) and total flavonoid content (TFC) were also measured by the Folin-Ciocalteu and spectrophotometry methods, respectively. The results indicated that the extracts from these two microalgae possessed good antioxidant capacity. Analysis showed that most antioxidant performance indicators (TAC, DPPH, and RP) were positively correlated with the TPC of the extracts, suggesting that the phenolics might be the major components in C. cohnii and Schizochytrium sp., contributing to their antioxidative function. Therefore, the polar fractions of C. cohnii and Schizochytrium sp. could be further examined and considered for application in health products or cosmetics.
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