| 男子30km跑后尿液蛋白质组差异性表达的研究 |
| |
| 引用本文： | 林文弢,蔺海旗,杨玲,徐国琴,翁锡全.男子30km跑后尿液蛋白质组差异性表达的研究[J].天津体育学院学报,2020(1):7-12. |
| |
| 作者姓名： | 林文弢 蔺海旗 杨玲 徐国琴 翁锡全 |
| |
| 作者单位： | 广州体育学院;华南理工大学体育学院;韶关学院 |
| |
| 摘 要： | 目的:从蛋白质组学整体的角度,探讨30 km跑对尿液蛋白质组图谱差异性表达的影响,筛选对运动应激具有重要意义的目标蛋白。方法:以参加全国绿道长跑挑战赛30 km跑的10名男子为研究对象,分别留取运动前(晨)、后尿液,应用双向凝胶电泳确立运动前后尿液蛋白质组的差异性表达图谱,选取运动前后差异性表达量上调≥5倍和新增的蛋白点10个,用基质辅助激光解吸附电离串联飞行时间质谱仪进行质谱鉴定。结果:30 km跑前、后尿液2-DE图谱平均检测到蛋白质点(1011±243)和(1737±15)个,共获得差异性表达蛋白点110个,上调与下调各55个。其中运动后上调≥5倍的蛋白质点10个,新增点23个;运动后下调≥5倍的蛋白质点18个,消失点6个。对目标蛋白进行质谱分析,共鉴定出6个蛋白点,它们分别是锌α2-糖蛋白、白蛋白、维生素D结合蛋白、前列腺特异性抗原、β-肌动蛋白和本斯-琼斯蛋白,上述蛋白分别与机体能量代谢方式的转变、物质的转运、应激保护有关。结论:30 km跑引起尿液蛋白质组表达显著差异,ZAG、Alb、VDB、PSA、β-Actin、BJP的差异表达可作为运动营养补剂的筛选、运动负荷评价、运动性疲劳及肌损伤诊断的潜在目标蛋白;尿液蛋白图谱可作为运动员血液生物护照的补充用于反兴奋剂检测,实现监测无创化。
|
| 关 键 词： | 运动 尿液 蛋白质组学 双向凝胶电泳 质谱 |
A Proteomic Different Expression Study of Urinary Protein Changes in Male Athletes after 30 Km Running |
| |
| LIN Wentao,LIN Haiqi,YANG Ling,XU Guoqin,WENG Xiquan.A Proteomic Different Expression Study of Urinary Protein Changes in Male Athletes after 30 Km Running[J].Journal of Tianjin Institute of Physical Education,2020(1):7-12. |
| |
| Authors: | LIN Wentao LIN Haiqi YANG Ling XU Guoqin WENG Xiquan |
| |
| Institution: | (Guangzhou Sport University,Guangzhou 510500,China;School of PE,South China University of Technology,Guangzhou 510641,Chi na;Shaoguan University,Shaoguan 512005,China) |
| |
| Abstract: | Objective:The purpose of this study was to investigate the effect of a 30-km running on the urinary proteome profile using a global proteomic ap proach,to identify differentially expressed proteins and to screen for target proteins important for exercise-induced stress responses.Methods:In this study,10 males who participated in the National Greenway Long-Distance Running Challenge were selected as research subjects.The sporting event was a men’s 30-km race.Urine samples were collected from the 10 males before(in the morning)and after the race.Two-dimensional gel electrophoresis(2-DE)was performed to establish the expression profile of the urinary proteome before and after the race.Among the proteins differentially expressed before and after the race,those in which expression levels were upregulated by at least 5-fold after the race and proteins expressed only after the race were selected(a total of 10)and analyzed us ing MALDI-TOF-MS.Results:2-DE profiles of urine samples collected before and after the race were generated,and the average number of detectable protein spots was 1011±243 and 1737±15,respectively.A total of 110 differentially expressed protein spots were detected,among which 55 proteins were upregulated,and 55 proteins were downregulated.Of the 110 differentially expressed proteins,10 proteins were upregulated by at least 5-fold,and 23 proteins were only de tected after the race.In contrast,18 proteins were downregulated by at least 5-fold,and 6 proteins were no longer expressed after the race.The target proteins were analyzed by mass spectrometry,and a total of 6 proteins,including zincα2 glycoprotein,albumin,vitamin D binding protein,prostate specific antigen,β-actin and Bence Jones protein,which are related to changes in energy metabolism,the transport of substances and stress protection,were identified.Conclu sions:Significant changes were detected in the urinary protein profile of men after a 30-km race.The differential expressions of ZAG,VDB,β-Actin,Alb,PSA and BJP can be used as potential target proteins for the screening of sports nutrition supplements,exercise load evaluation,exercise fatigue and muscle injury di agnosis.This may also complement the athletes′blood passport in doping control,to realize noninvasive in doping detection. |
| |
| Keywords: | exercise urine proteome two-dimensional gel electrophoresis mass spectrometry |
| 本文献已被 维普 等数据库收录！ |
|
