| 大鼠实验性胃溃疡期间大脑皮层SS免疫组织化学和原位杂交组织化学研究 |
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| 引用本文： | 王建伟,张耕,常燕,郑慧蛾,周济远.大鼠实验性胃溃疡期间大脑皮层SS免疫组织化学和原位杂交组织化学研究[J].河北北方学院学报(医学版),1999(4). |
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| 作者姓名： | 王建伟 张耕 常燕 郑慧蛾 周济远 |
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| 作者单位： | 张家口医学院组胚教研室 075000
(王建伟，张耕，常燕，郑慧蛾)，张家口医学院组胚教研室 075000(周济远) |
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| 摘 要： | 目的 观察大鼠实验性胃溃疡期间大脑皮层生长抑素免疫反应(SS-ir)和SSmRNA神经元的变化。方法 免疫细化PAP法;地高辛标记反意SScRNA探针mRNA原位杂交组化法。结果 正常大鼠SS-ir神经元广泛分布于大脑皮层。溃疡术后1d,大脑皮层纹状皮质区各层SS-ir神经元减少,但可见大量串珠样SS强阳性纤维,溃疡组SS-ir神经元数与盐水组相比无差异;术后4d,溃疡组大脑皮层纹状皮质区各层SS-ir神经元密集、数量明显增多,与盐水组、正常组相比均有差异(P<0.05,P<0.01或P<0.001)。术后10和23d,以上核区SS-ir神经元数仍高于盐水组和正常组(P<0.01,P<0.001)。术后4d,溃疡组大脑皮层SSmRNA神经元数量明显多于盐水组(P<0.001)。结论 大鼠实验性胃溃疡时期,大脑皮层SS-ir和SSmRNA神经元可能参与了胃溃疡自愈过程的调节。
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| 关 键 词： | 实验性胃溃疡 生长抑素 免疫组化 原位杂交组化 大脑皮层 |
STUDY ON THE PEPTIDE AND GENETIC EXPRESSIONS OF SOMATOSTATIN NEURONS IN THE RAT CEREBRAL CORTEX DURING EXPERIMENTAL GASTRIC ULCER |
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| Wang Jianwei Zhanggeng Chang Yan Zheng Hue Zhangyuan.STUDY ON THE PEPTIDE AND GENETIC EXPRESSIONS OF SOMATOSTATIN NEURONS IN THE RAT CEREBRAL CORTEX DURING EXPERIMENTAL GASTRIC ULCER[J].Journal of Hebei North University:Medical Edition,1999(4). |
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| Authors: | Wang Jianwei Zhanggeng Chang Yan Zheng Hue Zhangyuan |
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| Institution: | Wang Jianwei Zhanggeng Chang Yan Zheng Hue Zhangyuan |
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| Abstract: | Objective to observe the changes of SS-ir and SSmRNA neurons in the rat cerebral cortex during experimental gastric ulcer.Method Immunocyto-chemical PAP;In situ hybridization histochemistry with digoxigeniri-labelled amiserum SScRNA probe.Results SS-ir neurons were widely distributed in the normal rat cerebral cortex.On the 1st day after inducing ulcer,the SS-ir neuron count was decreased,but varicose SS-ir nerve fibers were dense and marked in striate cortex as compared with the normal control group,and there was no significant difference between experimental gastric ulcer group and saline control group.On the 4th day after operation in experimental gas trie ulcer group.In experimental gastric ulcer group,the SS-ir neuron counts were raised on the 4th,10th and 23rd days and the highest on the 4th day in striate cortex,and showen higher than those of saline control group and normal control group(P<0.05,P<0.01 or P<0.001),respectively.On the 4th day after inducing ulcer,the SSmRNA neuron count of experimental gastric ulcer group were higher than those of saline control group in striate cortex (p<0.001).Conclusion The cerebral cortex SS-ir and SSmRNA neuron counts showed obvious changes during self healing process of the experimental gastric ulcer.The results suggested that these cells participated in regulating gastric ulcer formation and self-healing process. |
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| Keywords: | Experimental gastric ulcer Somatostatin Immunocytochemistry In situhybridization histochem-stry Cerebral cortex |
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