Experimental therapeutic intervention with alpha tocopherol in ethanol induced testicular injuries in rats |
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| Authors: | M Maneesh Sanjiba Dutta Amit Chakrabarti D M Vasudevan |
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| Institution: | (1) Department of Psychiatry, Sikkim Manipal Institute of Medical Sciences, 737 102 Gangtok;(2) Department of Pharmacology, Sikkim Manipal Institute of Medical Sciences, 737 102 Gangtok;(3) Department of Biochemistry, Amrita Institute of Medical Sciences, 662 026 Kochi, India;(4) Department of Biochemistry, Melaka Manipal Medical College, 576 104 Manipal, India |
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| Abstract: | Infertility is well-established harmful effect in chronic alcoholism and so far, there is no effective treatment for this
condition. The study was conducted to determine the effects of alpha tocopherol on ethanol induced testicular injuries in
male albino rats of Wistar strain. Five groups (n=6) of animals were used. Group I served as control. Group II received daily
1.6g ethanol/kg body weight/day for 4 weeks orally. Group III received 1.6g ethanol+80mg alpha tocopherol/kg body weight/day
for four weeks orally. Group IV received 1.6g ethanol/kg body weight for/day 4 weeks and followed by 80mg alpha tocopherol/kg
body weight/day for four weeks orally. Group V received 1.6g ethanol/kg body weight/day orally for 4 weeks, followed by 4
weeks abstinence. Twently-four hours after the last treatment the rats were sacrificed using anesthetic ether. Testes were
removed and used for the estimation of extent of lipid peroxidation and tissue levels of antioxidants and steroidogenic enzymes.
Alpha tocopherol treatment increased the activities of testicularΔ
5, 3β-HSD. Moreover, the treatment was also associated with significant decrease in testicular oxidative stress. Ethanol-induced
oxidative stress and decreased steroidogenesis can be reversed by treatment with alpha tocopherol. |
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| Keywords: | Ethanol Alpha tocopherol Oxidative stress Steroids Testes |
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