| RR基因沉默对WSSV病毒复制的影响 |
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| 引用本文： | 陈朝阳,史鑫娜,郑丽燕,吴文林.RR基因沉默对WSSV病毒复制的影响[J].泉州师范学院学报,2014(2):17-19. |
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| 作者姓名： | 陈朝阳 史鑫娜 郑丽燕 吴文林 |
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| 作者单位： | 泉州师范学院化学与生命科学学院,福建泉州362000 |
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| 基金项目： | 国家自然科学基金资助项目（31072236）;泉州市科技局资金资助项目（2011215）. |
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| 摘 要： | 对虾白斑综合症病毒（WSSV）编码与核酸合成代谢相关的核糖核苷酸还原酶（Ribonucleotide Reductases，RR），与病毒DNA的复制有关．利用克隆技术将RR基因克隆到L4440载体，构建体内合成dsRNA的大肠杆菌HTn5工程菌．诱导该工程菌合成了RR基因的特异双链RNA（RR—dsRNA）和非特异双链RNA（gfP—dsRNA），分别与WSSV混合共注射凡纳滨对虾，在感染72h后用病毒检测试剂盒提取DNA模板用于荧光定量PCR分析，结果显示RR—dsRNA能有效抑制WSSV病毒粒子的增值．
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| 关 键 词： | RR基因 双链RNA WSSV |
RR Gene Silence Inhibited White Spot Syndrome Virus proliferation |
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| CHEN Zhao-yang,SHI Xin-na,ZHEN Li-yan,WU Wen-lin.RR Gene Silence Inhibited White Spot Syndrome Virus proliferation[J].Journal of Quanzhou Normal College,2014(2):17-19. |
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| Authors: | CHEN Zhao-yang SHI Xin-na ZHEN Li-yan WU Wen-lin |
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| Institution: | (School of Chemistry and Life Science, Quanzhou Normal University, Fujian 362000 ,China) |
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| Abstract: | Ribonucleotide reduetases （RR） is an important enzyme during synthesis.In this research, we used RNAi technology to silence RR gene and study the effect of RR gene silence on the WSSV proliferation.We synthesized RR gene specific double-stranded RNA （RR-dsRNA） in vivo by cloning the RR gene into the L4d40 plasmid and inducing dsRNA expression in E.coli HT115.RR-dsRNA was mixed with WSSV and injected into templates were extracted with virus that RR-dsRNA effectively inhibited shrimp Litopenaeus vannamei.72 h post-Infection,WSSV DNA detection kit for quantitative PCR analysis. The results showed WSSV proliferation. |
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| Keywords: | RR ds-RNA WSSV |
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