| HPLC法同时测定珠芽蓼中没食子酸、绿原酸和槲皮素成分的含量 |
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| 引用本文： | 李居安,王丽,杨天寿.HPLC法同时测定珠芽蓼中没食子酸、绿原酸和槲皮素成分的含量[J].宁夏师范学院学报,2014,35(3):74-77. |
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| 作者姓名： | 李居安 王丽 杨天寿 |
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| 作者单位： | 宁夏固原市药品检验所,宁夏固原,756000 |
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| 基金项目： | 宁夏自然科学基金资助项目 |
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| 摘 要： | 建立HPLC法同时测定珠芽蓼中没食子酸、绿原酸和槲皮素的方法.采用phenomenex Luna C18(2)色谱柱(4.6 mm×250 mm,5μm),柱温40℃,以1%冰醋酸水溶液-1%冰醋酸甲醇溶液为流动相,梯度洗脱,检测波长分别为272 nm(检测没食子酸)、350 nm(检测绿原酸、槲皮素),流速为0.8 mL·min-1.结果表明没食子酸、绿原酸及槲皮素分别在0.2568μg~1.712μg(r=0.9992)、0.2898μg~1.932μg(r=0.9995)、0.01772μg~0.1181μg(r=0.9993)范围内与峰面积呈良好线性关系,平均加样回收率(n=6)分别为98.2%(RSD为2.28%)、101.6%(RSD为2.39%)、100.6%(RSD为1.02%).本方法操作简单、结果准确,具有较好的重复性和稳定性,适用于珠芽蓼药材中3个有效成分的含量测定.
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| 关 键 词： | 珠芽蓼 没食子酸 绿原酸 槲皮素 高效液相色谱 |
Simultaneous Determination of Gallic Acid,Chlorogenic Acid and Quercetin in Polygonum Viviparum.L by HPLC |
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| LI Juan,WANG Li,YANG Tianshou.Simultaneous Determination of Gallic Acid,Chlorogenic Acid and Quercetin in Polygonum Viviparum.L by HPLC[J].Journal of Ningxia Teachers College,2014,35(3):74-77. |
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| Authors: | LI Juan WANG Li YANG Tianshou |
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| Institution: | (Institution for Drug Control of Guyuan City, Guyuan,Ningxia 756000) |
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| Abstract: | A method based on HPLC was developed to Simultaneously determine the content of gallic acid,chlorogenic acid,quercetin in Polygonum viviparum. L.. Phenomenex Luna C18（2） column（250 mm × 4. 6 mm,5 μm） was used and eluted at40 ℃. With a mobile of 1% acetic acid aqueous solution and 1% acetic acid methanol solution with gradient elution,At the flow rate of 0. 8 mL·min^-1. The detection wavelength was set at 272 nm（gallic acid） and 350 nm（chlorogenic acid and quercetin.）The linear range of gallic acid,chlorogenic acid and quercetin were 0. 2568 μg ~ 1. 712 μg（r = 0. 9992）,0. 2898 μg ~ 1. 932 μg（r =0.9995）,0.01772 μg ~ 0. 1181 μg（r = 0. 9993）,and spiked recoveries（n = 6） were 98. 2%（RSD2. 28%）,101. 6%（RSD2.39%）,100.6%（RSD1.02%）. The method is accurate,sensitive,reproducible,reproducible,suitable for the determination of the three bioactive constituents in the Polygonum viviparum. L |
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| Keywords: | Polygonum viviparum L Gallic acid Chlorogenic acid Quercetin HPLC |
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