| 6-甲基-2-（α-羟甲基）-1H-苯并咪唑荧光增敏探针法测定蛋白质 |
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| 引用本文： | 李芳,陈定奔,宋美芳,贾文平.6-甲基-2-（α-羟甲基）-1H-苯并咪唑荧光增敏探针法测定蛋白质[J].台州学院学报,2008,30(6):34-37. |
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| 作者姓名： | 李芳 陈定奔 宋美芳 贾文平 |
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| 作者单位： | 1. 台州学院,医药化工学院,浙江,临海,317000
2. 浙江台州医院,浙江,临海,317000 |
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| 基金项目： | 浙江省教育厅资助项目
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| 摘 要： | 以6-甲基-2-（α-羟甲基）-IH-苯并咪唑（MHMBM）作为荧光探针，利用蛋白质对MHMBM荧光增敏作用，建立了测定人血清总蛋白的新方法。结果表明，在中性水溶液中，MHMBM的荧光强度与牛蛋白（BSA）的浓度呈良好的线性关系，相关系数（r）为0．9995，检出限2．3μg／mL，线性范围4．6—60．0μg／mL。本方法用于测定两种样品溶液中的总蛋白，RSD（n=6）分别为1．7％和1．3％，回收率95．5％和98．6％。
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| 关 键 词： | 6-甲基-2-(α-羟甲基)-IH-苯并咪唑 荧光探针 人血清蛋白质 牛血清白蛋白 |
Fluorimetric Enhancement Determination of Protein with 6-methyl-2-(hydroxymethyl)-1H-benzimidazole as a Fluorescent Probe |
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| LI Fang,CHEN Ding-ben,SONG Mei-fang,JIA Wen-ping.Fluorimetric Enhancement Determination of Protein with 6-methyl-2-(hydroxymethyl)-1H-benzimidazole as a Fluorescent Probe[J].Journal of Taizhou University,2008,30(6):34-37. |
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| Authors: | LI Fang CHEN Ding-ben SONG Mei-fang JIA Wen-ping |
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| Institution: | LI Fang1,CHEN Ding-ben1,SONG Mei-fang2,JIA Wen-ping1 |
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| Abstract: | A new method for determination of total protein in human serum is developed based on the fluorescence enhancement of 5-methyl-2-（α-hydroxymethyl）-IH-benzimidazole （MHMBM）by bovine serum albumi （BSA）with the MHMBM as a novel fluorescence probe. The results show that the linear relationship between the fluorescence enhancement of MHMBM and the concentration of BSA is good in neutral aqueous solutions （pH=7.2）. The correlation coefficient（r） and the linear range are 0.9995 and 4.5-50.0μg/mL respectively with the detection limit of 2.3μg/mK The method has been applied to determination of total protein in two samples solution with 1.7% and 1.3% RSD（n=6）. The recovery are 95.5% and 98.6% respectively. |
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| Keywords: | 6-methyl-2-(hydroxymethyl)-1H-benzimidazole fluorescence probe HSA BSA |
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