bmo-miR-0001 and bmo-miR-0015 down-regulate expression of <Emphasis Type="Italic">Bombyx mori</Emphasis> fibroin light chain gene in vitro |
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| Authors: | Chen Chen Yang-yang Fan Xin Wang Fei Song Tao Jiang Ping Qian Shun-ming Tang Xing-jia Shen |
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| Institution: | 1.Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang,China;2.Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang,China |
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| Abstract: | Based on bioinformatic analysis, we selected two novel microRNAs (miRNAs), bmo-miR-0001 and bmomiR-0015, from high-throughput sequencing of the Bombyx mori larval posterior silk gland (PSG). Firstly, we examined the expression of bmo-miR-0001 and bmo-miR-0015 in 12 different tissues of the 5th instar Day-3 larvae of the silkworm. The results showed that the expression levels of both bmo-miR-0001 and bmo-miR-0015 were obviously higher in the PSG than in other tissues, implying there is a spatio-temporal condition for bmo-miR-0001 and bmo-miR-0015 to regulate the expression of BmFib-L. To test this hypothesis, we constructed pri-bmo-miR-0001 expressing the plasmid pcDNA3.0 ie1-egfp-pri-bmo-miR-0001-SV40] and pri-bmo-miR-0015 expressing the plasmid pcDNA3.0 ie1-egfp-pribmo-miR-0015-SV40]. Finally, the BmN cells were harvested and luciferase activity was detected. The results showed that luciferase activity was reduced significantly (P<0.05) in BmN cells co-transfected by pcDNA3.0 ie1-egfp-pri-bmomiR-0001-SV40] or pcDNA3.0 ie1-egfp-pri-bmo-miR-0015-SV40] with pGL3.0 A3-luc-Fib-L-3′UTR-SV40], suggesting that both bmo-miR-0001 and bmo-miR-0015 can down-regulate the expression of BmFib-L in vitro. |
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| Keywords: | Bombyx mori MicroRNA bmo-miR-0001 bmo-miR-0015 BmFib-L Regulation of expression |
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