| 植物病毒抗血清的制备及IgG纯化与酶标技术研究 |
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| 引用本文： | 梁峰,刘秀花.植物病毒抗血清的制备及IgG纯化与酶标技术研究[J].商丘师范学院学报,1997(Z4). |
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| 作者姓名： | 梁峰 刘秀花 |
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| 作者单位： | 商丘师专生物系!河南商丘，476000 |
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| 摘 要： | 以烟草花叶病毒（TMv）为例研究了制备植物病毒抗血清的一般方法．以家兔和小白鼠为免疫动物，运用饱和硫酸接沉淀法制备出高效价的兔和瓦抗TMV血清，并运用先进的ELISA方法测定了抗血清中抗体IgG的效价；用醋酸纤维凝胶电泳技术分析了抗血清中抗体的lgG纯度，应用免疫双扩散法检测了提纯的IgG的生物活性．同时还运用成二醛一步法制出高结合率的IgG与过氧化氢酶（HRP）标记的抗体，形成了一整套制备抗血清、纯化IgG并对IgG进行酶标的技术，为植物病毒检测提供了快速简便的方法．
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| 关 键 词： | 抗血清 制备 免疫球蛋白 纯化 酶联免疫吸附试验 酶标抗体 |
A STUDY ON THE TECHNIQUES OF THE PREPARATION OF PLANT VIRUS ANTISERUM AND THE PURIFICATION OF IgG |
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| Liang Feng Liu Xiuhua.A STUDY ON THE TECHNIQUES OF THE PREPARATION OF PLANT VIRUS ANTISERUM AND THE PURIFICATION OF IgG[J].Journal of Shangqiu Teachers College,1997(Z4). |
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| Authors: | Liang Feng Liu Xiuhua |
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| Abstract: | We studed the techniques of the preparation of plant virus antiserum and the pu-rification of IgG. Useing rabbit and mouse as immune animals,TMV as a antigene,we pre-pared anti-TMV serum. Assayed this antiserum by enzyme linked immunosorbent assay(ELISA),we fund it had a high titre. From rabbit antiserm puri ficated the lgG with satura-tion (NH'),SO, solution preipitate method,and this pure IgG had a high purity and a high bi-ological vigour. Useing this pure IgG and horseradish peroxidase (HRP) we have had a en-zyme labeling antibody, and, such this antibody can widespreadly used in immunoenzyatictechniques about assays of plant virus. |
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| Keywords: | preparation antiserum purification IgG ELISA enzyme labeling antibody |
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