| 剑叶龙血树愈伤组织的诱导及其增殖培养研究 |
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| 引用本文： | 杨晓虹,侯思名,和凤美,郭丽红,陈子牛.剑叶龙血树愈伤组织的诱导及其增殖培养研究[J].昆明师范高等专科学校学报,2009,31(6):64-66. |
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| 作者姓名： | 杨晓虹 侯思名 和凤美 郭丽红 陈子牛 |
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| 作者单位： | [1]昆明学院生命科学与技术系,云南昆明650031 [2]云南农业大学同林园艺学院,云南昆明650201 |
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| 基金项目： | 国家发展和改革委员会现代中药产业化专项基金 |
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| 摘 要： | 以西双版纳可提取药用活性成分“血竭”的剑叶龙血树为材料，进行愈伤组织的诱导及增殖培养，结果显示：外植体的消毒侧芽以75％乙醇浸泡2min＋0．1％升汞浸泡8min，花蕾以75％乙醇浸泡1．5min＋0．1％升汞浸泡8min，叶片以75％乙醇浸泡3min＋0．1％升汞浸泡12min效果较好；愈伤组织诱导以花蕾为外植体效果较好，且诱导与增殖的培养基为B5＋2．0mg/L NAA＋0．5mg／L6-BA．
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| 关 键 词： | 龙血树 血竭 愈伤组织 组织培养 |
The Callus Induction and Proliferation of Dracaena cochinchinensis |
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| YANG Xiao-hong,HOU Si-ming,HE Feng-mei,GUO Li-hong,CHEN Zi-niu.The Callus Induction and Proliferation of Dracaena cochinchinensis[J].Journal of Kunming Teachers College,2009,31(6):64-66. |
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| Authors: | YANG Xiao-hong HOU Si-ming HE Feng-mei GUO Li-hong CHEN Zi-niu |
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| Institution: | 1. Life Scienee anti Technology Department, Kunming University, Yunnan Kunming 650031, China; 2. Horticultural Institute, Yunnan Agricultural University, Yunnan Kunming 650201, China) |
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| Abstract: | The induction and proliferation of callus is done by using the plant from Xishuangbanna Dracaena cochinchinensis from which extracted medicinal active ingredients ＂Resina Draconis＂ as the material. The results showed that 2 min soaking in 75% ethanol plus 8min in 0.1% HgCl2 were the best sample treatment for buds, the same as 1.5 min in 75% ethanol plus 8 min in 0.1% HgCl2 for budet and 3min in 75% ethanol plus 12min in 0. 1% HgCl2 for leaves. The budet is the best material for callus induction of D. cochinchinensis and the optimal culture media to induction and proliferation is B5 ＋ 2.0 mg/L NAA ＋ O. 5 mg/L 6 -BA. |
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| Keywords: | Dracaena cochinehbvensis tesina draconis callus tissue culture |
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