| DNA分子标记技术在水生动物遗传多样性研究中的应用 |
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| 引用本文： | 王忠华,董西征,钱国英.DNA分子标记技术在水生动物遗传多样性研究中的应用[J].科技通报,2008,24(5). |
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| 作者姓名： | 王忠华 董西征 钱国英 |
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| 作者单位： | 浙江万里学院生物技术研究所,浙江,宁波,315100 |
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| 摘 要： | 简要阐述了几种常见DNA分子标记技术,如限制性片断长度多态性(restriction fragment length polymorphism,RFLP)、随机扩增多态性DNA(random amplified polymorphie DNA,RAPD)、扩增片断长度多态性(amplified fragment length polymorphism,AFLP)、简单重复序列或微卫星标记(simpie sequencerepeat,SSR)、内部简单重复序列(inter-simple sequence repeat,ISSR)和单核苷酸多态性(single nucleotidepelymorphisms,SNP)等的基本原理、技术上的优缺点及其在水生动物遗传多样性研究中的应用,包括遗传多样性的鉴定、品种亲缘关系与分类的研究和种质资源的鉴定等.最后,对DNA分子标记技术在水生动物遗传多样性应用中的存在问题进行了简单的讨论.
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| 关 键 词： | DNA分子标记 水生动物 遗传多样性 应用 |
Application in Genetic Diversity of Aquatic of DNA Molecular Markers |
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| WANG Zhonghua,DONG Xizheng,QIAN Guoying.Application in Genetic Diversity of Aquatic of DNA Molecular Markers[J].Bulletin of Science and Technology,2008,24(5). |
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| Authors: | WANG Zhonghua DONG Xizheng QIAN Guoying |
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| Abstract: | The basic principle, advantages and disadvantages of several DNA molecular markers technology, such as restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism(AFLP), simple sequence repeat(SSR), inter-simple sequence repeat(ISSR)and single nucleotide polymorphisms(SNPs)etc. and its application in aquatic, including the identification of genetic diversity, the investigation of relationship and classification of varieties belonged to same species, identification of germplasm, etc were briefly reviewed. Finally, the problems existing in the application of DNA molecular markers in aquatic were briefly discussed. |
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| Keywords: | DNA molecular markers Aquatic genetic diversity application |
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