| Nrf2抵抗胰岛β细胞氧化损伤的作用机制 |
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| 作者姓名： | 宁梦丽 丁文军 张芳 |
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| 作者单位： | 中国科学院大学生命科学学院, 北京 100049 |
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| 基金项目： | 国家自然科学基金(11075207,21377127)和中国科学院知识创新工程重大项目(KSCX2-EW-J-29)资助 |
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| 摘 要： | 长期高血糖导致糖尿病患者的氧化应激,引起胰岛β细胞氧化损伤,但核转录因子Nrf2(NF-E2-related factor 2)抵抗胰岛β细胞氧化损伤的作用机制还不清楚.本研究用低浓度葡萄糖(LG,5.6 mmol/L)、LG+H2O2和高浓度葡萄糖(HG,27.6 mmol/L)分别处理小鼠胰岛NIT-1β细胞48 h,检测细胞内活性氧(ROS,reactive oxygen species)生成、胰岛素合成与分泌变化和Nrf2入核表达水平.研究发现,高糖诱导NIT-1β细胞的ROS生成,降低细胞合成与分泌胰岛素的水平,但Nrf2入核表达降低胰岛β细胞氧化应激.结果提示Nrf2入核表达可以抵抗高糖诱导的胰岛β细胞氧化损伤,改善细胞合成与分泌胰岛素的功能.
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| 关 键 词： | 高糖 胰岛&beta 细胞 氧化应激 Nrf2 |
| 收稿时间： | 2014-05-07 |
| 修稿时间： | 2014-05-28 |
Cytoprotective effect of Nrf2 expression on hyperglycemia-induced oxidative stress in pancreatic NIT-1β-cells |
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| Authors: | NING Mengli DING Wenjun ZHANG Fang |
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| Institution: | College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | It is well known that hyperglycemia results in oxidative stress in diabetes mellitus, which causes pancreatic β cell dysfunction. However, this cytoprotective effect of Nrf2 on hyperglycemia-induced oxidative stress in pancreatic β cell is not fully understood. In the study, reactive oxygen species (ROS) and insulin synthesis and secretion as well as nucleus NF-E2-related factor 2 (Nrf2) expression in NIT-1β cells were determined after treatment of 48 h with low glucose (LG, 5.5 mmol/L), high glucose (HG, 27.6 mmol/L), or LG plus H2O2, respectively. We found that HG significantly induced ROS generation and decreased insulin synthesis and secretion in NIT-1β cells. However, HG-induced oxidative damage was improved by nucleus NF-E2-related factor 2 (Nrf2) expression. The results suggested that activation of Nrf2 through promotion of nuclear accumulation plays an important role in protection against hyperglycemia-induced oxidative injury. |
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| Keywords: | hyerglycemia pancreatic &beta cell oxidative stress Nrf2 |
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