| 不同持续时间低氧后训练对大鼠海马组织细胞凋亡的影响 |
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| 引用本文： | 张沙骆,瞿树林,陈嘉勤.不同持续时间低氧后训练对大鼠海马组织细胞凋亡的影响[J].天津体育学院学报,2008,23(1):34-38. |
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| 作者姓名： | 张沙骆 瞿树林 陈嘉勤 |
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| 作者单位： | 湖南师范大学体育学院,长沙,410012 |
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| 基金项目： | 湖南省自然科学基金
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湖南省教育厅科研项目 |
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| 摘 要： | 目的:观察不同持续时间低氧后训练对大鼠海马组织细胞凋亡的影响,探讨低氧训练对海马神经细胞凋亡的影响,为科学地指导运动员进行低氧训练提供实验依据.方法:雄性SD大鼠60只,随机分为6组,每组10只,正常对照组(A组),低氧8 h组(B组),低氧12h组(C组),训练对照组(D组),低氧 8 h 训练组(E组),低氧 12 h 训练组(F组).采用零坡度跑台的训练方式,对D、E和F 3组以25 m/min 的速度在常氧环境中每天训练1 h.将B、C、E和F组放人低氧舱内,氧浓度为 12.5%(相当于 4000 m 海拔高度),过8 h和12h后,分别将B、E组和c、F组取出放入正常氧浓度环境.训练共持续4周,每周5天.最后一次训练至力竭后 24 h 断头处死,取大鼠海马组织,测定Bax和BcI-2蛋白表达的阳性细胞个数和凋亡指数.研究结果显示:在低氧训练过程机体对低氧刺激的适应性改变,使得在停止运动后,海马组织的损害减小.随着低氧时间的延长,低氧训练使大鼠海马组织CA1区的细胞凋亡有减少的趋势,从而起到神经保护作用.
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| 关 键 词： | 不同持续时间 低氧训练 海马 细胞凋亡 持续时间 低氧舱 训练过程 大鼠海马组织 细胞凋亡 影响 Rats Apoptosis Hippocampus Time Training Different 神经保护作用 趋势 延长 损害 运动后 适应性 刺激 机体 |
| 文章编号： | 1005-0000(2008)01-0034-05 |
| 收稿时间： | 8/9/2007 12:00:00 AM |
| 修稿时间： | 2007年8月9日 |
The Effect of Different Hypoxic Training Time on Hippocampus Apoptosis of Rats |
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| ZHANG Shaluo,QU Shulin,CHEN Jiaqin.The Effect of Different Hypoxic Training Time on Hippocampus Apoptosis of Rats[J].Journal of Tianjin Institute of Physical Education,2008,23(1):34-38. |
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| Authors: | ZHANG Shaluo QU Shulin CHEN Jiaqin |
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| Institution: | School of PE, Hunan Normal University, Changsha 410012, China;School of PE, Hunan Normal University, Changsha 410012, China;School of PE, Hunan Normal University, Changsha 410012, China |
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| Abstract: | To examine the effect of hypoxic exercise on rat's hippocampus tissue apoptosis,and also examine the effect on hippocampus nerve apoptosis,which could provide an experimental record to scientific guidance of exercisers' hypoxic exercise.Method: We took 60 male SD rats as subjects,and randomly distributed them into 6 groups,10 rats each,which were normal contrast group(Group A),hypoxia 8-hour group(Group B),hypoxia 12-hour group(Group C),training contrast group(Group D),hypoxia 8-hour training group(Group E),and hypoxia 12-hour training group(Group F).Group D,E,F took 1 hour treadmill training in the rate of 25m/min in normal oxygen environment.On 8 o'clock next morning,we put B,C,E and F group into hypoxia cabin,oxygen concentration is 12.5%(equal to 4km height),8 and 12 hours later,put group B,E and C,F into normal oxygen concentration environment separately.This training lasted 4 weeks,and 5 days each week.24 hours after the last training to death,we took hippocampus tissue,proceeding with fixing and tissue embedding,using immunohisto chemistry and TUNEL to measure Bax,Bcl-2 and apoptosis index.Conclusion: Increasing the duration of hypoxic period,hypoxic exercise training decreased the apoptosis of cells in rat's hippocampus tissue CA1,which protects the nerves.In the hypoxic exercise,due to the change of body's adaptability to hypoxic stimulus,after exercise,the damage to hippocampus tissues also decreased. |
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| Keywords: | different lasting time hypoxic exercise hippocampus cell apoptosis |
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