| Science Letters:Construction of a eukaryotic expression plasmid of Humanin |
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| 作者姓名： | 罗本燕 陈祥明 唐敏 陈峰 陈智 |
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| 作者单位： | Department of Neurology，First Affiliated Hospital，School of Medicine，Zhejiang University，Hangzhou310003，China，Department of Anesthesiology，Second Affiliated Hospital，School of Medicine，Zhejiang University，Hangzhou310009，China，Department of Neurology，First Affiliated Hospital，School of Medicine，Zhejiang University，Hangzhou310003，China，Institute of Infectios Diseases.First Affiliated Hospital，School of Medicine，Zhejiang University，Hangzhou310001.China，Institute of Infectios Diseases.First Affiliated Hospital，School of Medicine，Zhejiang University，Hangzhou310001.China |
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| 基金项目： | Project(No.2003C30040)
supportedbytheScienceandTechnologyDepartmentofZhejiangProvince,China |
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| 摘 要： | Objective:To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.Methods:The recombinant plasmidpGEMEX-1-Humanin was digested with restriction endonucleases BamH Ⅰ and Hind Ⅲ and the Humanin gene fragments,about100 bp length,were obtained.Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-)andthe recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.Results:Recombinant plasmid DNA success-fully produced a band which had the same size as that of the thimauin positive control.The sequence of recombinant plasmidsaccorded with the Humnain gene sequence.Conclusions:A eukaryotic expression plasmid of Humanin was successfully con-structed.
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Science Letters:Construction of a eukaryotic expression plasmid of Humanin |
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| LUO Ben-yan,CHEN Xiang-ming,TANG Min,CHEN Feng.Science Letters:Construction of a eukaryotic expression plasmid of Humanin[J].Journal of Zhejiang University Science,2005(1). |
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| Authors: | LUO Ben-yan CHEN Xiang-ming TANG Min CHEN Feng |
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| Institution: | LUO Ben-yan~1 CHEN Xiang-ming TANG Min CHEN Feng |
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| Abstract: | |
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| Keywords: | Humanin Alzheimer's disease Eukaryotic expression |
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