| Character of HBV （hepatitis B virus） polymerase gene rtM204V／I and rtL180M mutation in patients with lamivudine resistance |
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| 作者姓名： | Li MW Hou W Wo JE Liu KZ |
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| 作者单位： | Institute of Infectious Diseases,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China,Institute of Infectious Diseases,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China,Institute of Infectious Diseases,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China,Institute of Infectious Diseases,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China |
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| 摘 要： | Objectives: To investigate the relationship between HBV (hepatitis B virus) polymerase gene 180 and 204 sites mutation and lamivudine resistance. Methods: One hundred forty-one patients with lamivudine resistance after lamivudine treatment and 60 chronic hepatitis B patients without lamivudine treatment were enrolled in this study. The serum HBV DNA mutation was analyzed by sequence detection via polymerase chain reaction (PCR). The sequences of the same patient were analyzed before and after lamivudine treatment. Results: One hundred and nine lamivudine resistance patients had HBV YMDD (tyrosine-methionine-aspartate-aspartate) mutation. Among them, 45 patients had rtL180M/M204V mutation (41.28%), 28 patients had rtL 180M/M204I mutation (25.70%) and 36 patients had rtM204I mutation (33.02%). There were 6 patients with rtL180M mutation in 32 lamivudine resistance patients. Sixty chronic hepatitis patients without lamivudine treatment had no mutations. Conclusions: HBV mutations, which play an important role in lamivudine resistance usually locate at polymerase gene 204 site; 180 site mutation was also observed in these patients. Evaluation of the anti-virus therapy by surveillance of the two sites mutations is of importance.
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| 关 键 词： | 乙型肝炎病毒 rtM204V／I rtL180M 病毒感染 基因表达 基因突变 |
Character of HBV (hepatitis B virus) polymerase gene rtM204V/I and rtL180M mutation in patients with lamivudine resistance |
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| Li MW,Hou W,Wo JE,Liu KZ.Character of HBV (hepatitis B virus) polymerase gene rtM204V/I and rtL180M mutation in patients with lamivudine resistance[J].Journal of Zhejiang University Science,2005,6(7):664-667. |
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| Authors: | Li Min-wei Hou Wei Wo Jian-er Liu Ke-zhou |
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| Institution: | Institute of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. limw@zju.edu.cn |
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| Abstract: | Objectives: To investigate the relationship between HBV (hepatitis B virus) polymerase gene 180 and 204 sites mutation and lamivudine resistance. Methods: One hundred forty-one patients with lamivudine resistance after lamivudine treatment and 60 chronic hepatitis B patients without lamivudine treatment were enrolled in this study. The serum HBV DNA mutation was analyzed by sequence detection via polymerase chain reaction (PCR). The sequences of the same patient were analyzed before and after lamivudine treatment. Results: One hundred and nine lamivudine resistance patients had HBV YMDD (tyrosine-methionine-aspartate-aspartate) mutation. Among them, 45 patients had rtL 180M/M204V mutation (41.28%), 28patients had rtL180M/M204I mutation (25.70%) and 36 patients had rtM204I mutation (33.02%). There were 6 patients with rtL180M mutation in 32 lamivudine resistance patients. Sixty chronic hepatitis patients without lamivudine treatment had no mutations. Conclusions: HBV mutations, which play an important role in lamivudine resistance usually locate at polymerase gene 204 site; 180 site mutation was also observed in these patients. Evaluation of the anti-virus therapy by surveillance of the two sites mutations is of importance. |
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| Keywords: | Hepatitis B virus Lamivudine YMDD mutant Sequence analysis |
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