| 低氧运动对骨骼肌mTOR/p70~(S6K)通路的影响 |
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| 引用本文： | 赵华,曾凡星,张漓.低氧运动对骨骼肌mTOR/p70~(S6K)通路的影响[J].北京体育大学学报,2009(12). |
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| 作者姓名： | 赵华 曾凡星 张漓 |
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| 作者单位： | 天水师范学院体育学院;北京体育大学;国家体育总局体育科学研究所; |
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| 基金项目： | 国家自然科学基金项目(批准号30671013) |
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| 摘 要： | 目的:研究4周低氧运动中mTOR/p70S6K通路的变化,以探讨低氧训练中的蛋白合成代谢情况。方法:以12周龄雄性SD大鼠为研究对象,分为常氧安静组(NS组)、常氧运动组(NE组)、低氧安静组(HS组)和低氧运动组(HE组)。采用动物跑台训练,低氧刺激为常压低氧(氧浓度13.6%,相当于3 500 m海拔)。检测大鼠趾长伸肌的总蛋白含量及mTOR、p70S6K和p70S6K(Thr389)磷酸化表达。结果:NE组和HE组mTOR蛋白表达均显著高于HS组。NE组p70S6K蛋白表达显著高于NS组和HS组。NE组p70S6K(Thr389)磷酸化显著高于HS组和HE组,NS组显著高于HS组和HE组,HE组显著高于HS组。结论:1)低氧抑制肌肉蛋白合成,常氧运动促进肌肉蛋白合成。2)长期低氧暴露抑制mTOR/p70S6K表达,运动促进表达,低氧运动削弱了低氧对mTOR/p70S6K表达的抑制作用。
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| 关 键 词： | 低氧运动 蛋白合成 mTOR p70S6K |
Effects of Hypoxic Exercise on mTOR/p70~(S6K) Signaling Pathway in Skeletal Muscle |
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| ZHAO Hua,ZENG Fan-xing,ZHANG Li.Effects of Hypoxic Exercise on mTOR/p70~(S6K) Signaling Pathway in Skeletal Muscle[J].Journal of Beijing Sport University,2009(12). |
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| Authors: | ZHAO Hua ZENG Fan-xing ZHANG Li |
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| Institution: | ZHAO Hua1,ZENG Fan-xing2,ZHANG Li3(1.P.E.Department,Tianshui Normal University,Tianshui 741001,Gansu China,2.Beijing Sport University,Beijing 100084,China,3.National Institute of Sport Science,Beijing 100061,China) |
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| Abstract: | Objective:The present paper is to study the change of mTOR/p70S6K pathway in 4 weeks hypoxic exercise.Methods: Adult male Sprague-Dawley rats(12 weeks old) are randomly divided into four groups: NS,NE,HS and HE.The hypoxia circumstance is normal pressure hypoxia,with oxygen concentration of 13.6%,equivalent to 3500m above sea level.Total protein content,mTOR and p70S6K total protein,p70S6K(Thr389) phosphorylation of extensor digitorum longus (EDL) muscle are tested by Bradford and Western blot.Results: Tota... |
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| Keywords: | hypoxic exercise protein synthesis mTOR p70S6K |
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