| 碱性苦味酸法和酶法测血清肌酐的方法学比较 |
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| 引用本文： | 马恩龙,石宏宴,董枫.碱性苦味酸法和酶法测血清肌酐的方法学比较[J].大连大学学报,2004,25(6):53-55. |
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| 作者姓名： | 马恩龙 石宏宴 董枫 |
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| 作者单位： | 1. 大连市卫生学校,辽宁,大连,116622
2. 大连医科大学,辽宁,大连,116027 |
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| 摘 要： | 目的]了解酶法和碱性苦味酸法测定血清肌酐结果的偏倚大小,对两种方法进行比较.方法]用生化仪器OLYMPUSAU1000分别进行酶法和碱性苦味酸法测定血清肌酐.对两种方法进行重复性实验、相关性实验、线性比较和回收试验.结果]酶法和碱性苦味酸法的重复性实验数据(x±s)266±2.77μmol／L和273.5±3.30μmol／L,其变异系数分别为1.04％、1.21％;两者的回归方程式yJaffe=1.01X二步酶法-1.28;酶法的线形范围远大于碱性苦味酸法;在回收试验中两者的回收率分别为98.5％、94.5%.结论]酶法和苦味酸法有较好的相关性,酶法比碱性苦味酸法线性范围宽,特异性和灵敏度高,测定重复性好,是比较理想的方法.临床上酶法将逐渐代替苦味酸法测定血清肌酐.
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| 关 键 词： | 肌酐 酶法 碱性苦味酸法 |
| 文章编号： | 1008-2395(2004)06-0053-03 |
| 修稿时间： | 2004年9月26日 |
Comparison of determining the sero-creatinine by enzymatic assay and jaffe assay |
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| MA En-long,SHI Hong-yan,DONG Feng.Comparison of determining the sero-creatinine by enzymatic assay and jaffe assay[J].Journal of Dalian University,2004,25(6):53-55. |
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| Authors: | MA En-long SHI Hong-yan DONG Feng |
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| Abstract: | Objective ] To determine the relative bias between Enzymatic assay and Jaffe assay by the sero-creatinine (CREA) evaluation of the fresh patient samples. Methods ] Enzymatic assay and Jaffe assay were studied by Olympus AU1000 to evaluate sero-creatinine. The two assays were compared by reproducibility test,dependability test,linearity test and recovery test. Results]The reproducibility test datum of Enzymatic assay and Jaffe assay were 266±2.77μmol/L and 273.5±3. 30 μmol/L. The Variation coefficient were 1.04% and 1.21% respectively. The coefacient of correlation was calculated for two the methods, Y Jaffe= 1. 01 X Emymalic - 1. 28. The recovery rates of Enzymatic assay and Jaffe assay were 98.5% and 94. 5%. Conclusions] Enzymatic assay of analyzing the sero-creatinine is simple sensitive and suitable to apply to clinical. |
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| Keywords: | Sero-creatinine Enzymatic assay Jaffe assay |
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