| Effects of angiotensin Ⅱ on connexin 43 of VSMCs in arteriosclerosis |
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| 作者姓名： | CAI Wei RUAN Li-ming WANG Yi-na CHEN Jun-zhu |
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| 作者单位： | Department of Internal Medicine, the First Affiliated Hospital School of Medicine, Zhejiang University, Hangzhou 310003, China |
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| 摘 要： | Objective： To observe the effect ofangiotensin Ⅱ （Ang Ⅱ） on expression of gap junction channel protein connexin 43 （Cx43） in the proliferation process of vascular smooth muscle cells （VSMCs） during the early stage of arteriosclerosis. Methods： Thirty-two adult male rabbits were randomly divided into 4 groups. Rabbits in Group A were fed common diet while others in Groups B, C, and D were fed high-cholesterol diet. Losartan （10 mg/（kg.d）） and ramipril （0.5 mg/（kg-d）） were added in the diet of Groups C and D, respectively. The animals were sacrificed after 8 weeks and abdominal aortas were removed and dissected. The expression of Cx43 was assayed using RT-PCR and Western Blotting analysis. Results： Cx43 was increased markedly in both protein and mRNA level in Groups B, C, and D fed high-cholesterol diet compared with that in control group （P〈0.01）. Cx43 level in losartan or ramipril treated groups was higher than that in control group （P〈0.01, P〈0.05）, but lower than that in high-cholesterol diet groups （P〈0.05, P〈0.01）. Conclusion： Cx43 level was upregulated in VSMCs during early atherosclerosis. Losartan and ramipril can inhibit the expression of Cx43.
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| 关 键 词： | 动脉硬化症 连接蛋白 mRNA VSMCs 血管紧缩素 |
| 收稿时间： | 2005-10-11 |
| 修稿时间： | 2006-02-14 |
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