| 不同处理条件对洋葱鳞茎表皮细胞骨架形态影响的研究 |
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| 引用本文： | 马云,张妍妍,王启钊,王伍,张巧玉,陈改红.不同处理条件对洋葱鳞茎表皮细胞骨架形态影响的研究[J].茂名学院学报,2009,19(3):15-19. |
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| 作者姓名： | 马云 张妍妍 王启钊 王伍 张巧玉 陈改红 |
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| 作者单位： | 信阳师范学院,生命科学学院,河南,信阳,464000 |
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| 基金项目： | 河南省自然科学基金项目(082300450490);;河南省教育厅高等学校青年骨干教师资助计划;;信阳师范学院青年骨干教师资助计划(2006BAD04A16) |
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| 摘 要： | 细胞骨架是活细胞的重要结构，研究细胞骨架的形态对于进一步研究其功能具有重要意义。本研究以洋葱为材料，设置了4个不同的1％Triton X-100抽提非骨架蛋白时间（10min、15min、25min、40min）对洋葱细胞骨架显微观察的关键条件进行了优化，同时分析了细胞环境温度（-18℃、4℃、20℃、50℃），紫外线照射活细胞（0min、60min），0．1mol／L Ca^2＋处理及0．1％秋水仙素处理对洋葱细胞骨架形态观察效果的影响。结果表明：1％Triton X-100抽提非骨架蛋白25min观察效果较好；-18℃、50℃，紫外线辐射60min，0．1mol／L Ca^2＋和秋水仙素处理均会影响细胞骨架的观察。
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| 关 键 词： | 细胞骨架 温度 紫外线 洋葱鳞茎 显微观察 |
On The Influence of Different Treatments for Cytoskeleton from Onion Inner - epidermal Cell |
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| MA Yun,ZHANG Yan-yan,WANG Qi-zhao,WANG Wu,ZHANG Qiao-yu,CHEN Gai-hong.On The Influence of Different Treatments for Cytoskeleton from Onion Inner - epidermal Cell[J].Journal of Maoming College,2009,19(3):15-19. |
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| Authors: | MA Yun ZHANG Yan-yan WANG Qi-zhao WANG Wu ZHANG Qiao-yu CHEN Gai-hong |
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| Institution: | College of Life Science;Xinyang Normal University;Xinyang 46400;China |
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| Abstract: | Cytoskeleton was an important construction of life cell, with significance to investigate its function by observing its form. In order to study the crucial factor of this experiment, four different time （ 10min, 15min, 25min, 40min） of extracting non - cytoskeleton protein was set to optimize microscopic observation of onion cytoskeleton. At the same time, influence factors of treating with different temperatures （ - 18℃, 4℃, 20℃, 50℃）and UV- B radiation （60min） , 0. 1%eolchicin processing and 0. 1mol/L Ca^2＋ processing were studied. Results showed that extracting non - cytoskeleton protein with 1%Triton X - 100 for 25min had a positive effect on the microscopic observation on cytoskeleton of onion inner - epidermal cell. Low temperature （ - 18℃ ）, high temperature （50℃）, UV - B radiation （60min）, 0.1%colchicin processing and 0. 1mol/L Ca^2＋ processing had a negative effect on cytoskeleton. |
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| Keywords: | cytoskeleton temperature UV-B radiation onion bulb microscopic observation |
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