| Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified gerbera |
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| 作者姓名： | Lai QX Bao ZY Zhu ZJ Qian QQ Mao BZ |
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| 作者单位： | [1]Key Laboratory of Horticultural Plant Development and Biotechnology, Department of Horticulture, Zhejiang University, Hangzhou 310029, China [2]Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China [3]Department of Horticulture, Zhejiang Forestry University, Lin＇an 311300, China |
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| 摘 要： | Leaf senescence is often caused by water deficit and the chimeric gene PSA612-1PT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance （TBARS） and antioxidant enzymes activities were investigated during leaf senescence of PSA612-1PT modified gerbera induced by osmotic stress compared with the control plant （wild type）. Leaf discs were incubated in 20%, 40% （w/v） polyethylene glycol （PEG） 6 000 nutrient solution for 20 h under continuous light 130 μmol/（m^2·s）]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase （SOD）, catalases （CAT）, ascorbate peroxidase （APX）, guaiacol peroxidase （GPX） and dehydroascorbate reductase （DHAR） were stimulated by PEG treatment. However, the increases were higher in PSA612-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation （TBARS content） was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities ofantioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress.
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| 关 键 词： | 大丁草 抗氧化剂 叶片质量 衰老 |
| 收稿时间： | 26 September 2006 |
| 修稿时间： | 2006-09-262007-02-08 |
| 本文献已被 CNKI 维普 PubMed SpringerLink 等数据库收录！ |
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