Molecular characterization of a defense-related AMP-binding protein gene, OsBIABP1, from rice |
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| Authors: | Xin-chun Zhang Xin Yu Hui-juan Zhang Feng-ming Song |
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| Institution: | (1) State Key Laboratory of Rice Biology, Department of Plant Protection, Institute of Biotechnology, Zhejiang University-Huajiachi Campus, 310029 Hangzhou, Zhejiang, People’s Republic of China; |
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| Abstract: | We cloned and characterized a rice gene OsBIABPI encoding an AMP-binding protein. The full-length cDNA of OsBIABP1 is 1912-bp long and is predicted to encode a 558-aa protein. OsBIABP1 contains a typical AMP-binding signature motif and shows high similarity to members of AMP-binding protein family. OsBIABP1 is expressed in stems, leaves and flowers of rice plants, but is not expressed, or expressed at a very low level, in rice roots. The expression of OsBIABP1 was induced by some defense-related signal molecules, e.g., salicylic acid (SA), benzothiadiazole, jasmonic acid (JA), and 1-amino cyclopropane-1-carboxylic acid, which mediate SA-and JA/ethylene (ET)-dependent defense signaling pathways, respectively. Furthermore, the expression of OsBIABP1 is activated by the infection of Magnaporthe oryzae, and the induced expression is quicker and stronger during early stages of pathogenesis in incompatible interaction than that in compatible interaction between rice and M. oryzae. Our results suggest that OsBIABP1 may be a defense-related AMP-binding protein that is involved in the regulation of defense re-sponse through SA and/or JA/ET signaling pathways. |
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| Keywords: | Rice Oryza sativa L Magnaporthe oryzae Defense response OsBIABP1 AMP-binding protein |
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