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| 狐米草低能重离子各诱变株系ITS序列比较 | |
| 引用本文： | 闫茂华,薛华杰,陆长梅,吴国荣,钦佩.狐米草低能重离子各诱变株系ITS序列比较[J].连云港师范高等专科学校学报,2007(2):74-76. |
| 作者姓名： | 闫茂华 薛华杰 陆长梅 吴国荣 钦佩 |
| 作者单位： | 1. 南京师范大学生命科学学院,江苏省资源生物技术重点实验室,江苏,南京,210097;连云港师范高等专科学校,初等教育系,江苏,连云港,222006 2. 南京师范大学生命科学学院,江苏省资源生物技术重点实验室,江苏,南京,210097 3. 南京师范大学生命科学学院,江苏省资源生物技术重点实验室,江苏,南京,210097;南京大学,生命科学学院,江苏,南京,210093 |
| 基金项目： | 江苏省科学技术委员会自然科学基金，江苏省重点实验室基金 |
| 摘 要： | 为了检验狐米草低能重离子各诱变株系在表型、生物量和内部生理生化状态发生变化的同时,其遗传物质DNA是否发生改变,试验检测了狐米草对照株系与各诱变株系的ITS序列.结果发现,所检测的狐米草10个诱变株系的ITS序列与对照株系完全一致,仅与美国德克萨斯州和路易斯安那州的狐米草株系差别1-2个碱基,显示低能重离子束诱变并没有导致狐米草株系在ITS区段发生变异. |
| 关 键 词： | 狐米草 低能重离子诱变 |
| 文章编号： | 1009-7740（2007）02-0074-03 |
| 修稿时间： | 2006-10-12 |
ITS Sequence Comparison among Strains of Spartina Patens Mutated by Low Energy Heavy Ion |
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| YAN Mao-hua,XUE Hua-jie,LU Chang-mei,WU Gu-rong,QIN Pei.ITS Sequence Comparison among Strains of Spartina Patens Mutated by Low Energy Heavy Ion[J].Journal of Lianyungang Teachers College,2007(2):74-76. | |
| Authors: | YAN Mao-hua XUE Hua-jie LU Chang-mei WU Gu-rong QIN Pei |
| Institution: | 1.Jiangsu Key Lab for Bioresource Technology, Nanjing Normal University, Nanjing 210097, China; 2.Lianyungang Teachers College, Lianyuangang 222006, China;3.Nanjing University, Nanjing 210093, China |
| Abstract: | Low energy heavy ion radiation has caused many changes in characters of shapes,biomasses and interior physiology etc among strains of Spartina patens.In order to test whether DNA sequences have also been changed,ITS sequences of 10 mutated strains and the control strains are assayed and compared in this paper.Results show that ITS sequences from these 11 strains are all the same,and they are different at 1-2 sites with strains from states of Texas and Louisiana of USA.These demonstrate that low energy heavy ion radiation has not caused variation in ITS section among these 10 mutated strains. |
| Keywords: | ITS |
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