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甘肃中华蟾蜍和中国林蛙核型的初步研究
引用本文:袁毅君,杨金义,袁惠君,杨晓亮.甘肃中华蟾蜍和中国林蛙核型的初步研究[J].天水师范学院学报,2007,27(5):26-28.
作者姓名:袁毅君  杨金义  袁惠君  杨晓亮
作者单位:1. 天水师范学院,生命科学与化学学院,甘肃,天水,741001
2. 天水师范学校,甘肃,天水,741001
3. 兰州理工大学,生命科学与工程学院,甘肃,天水,730050
摘    要:经酵母汁、秋水仙素对甘肃中华蟾蜍(Bufo gargarizans cantor)和中国林蛙(Rana chensinensis)处理后,取骨髓细胞制作染色体标本,对其核型分别进行了初步研究分析。结果表明,中华蟾蜍的二倍体染色体数目为2n=22,由6对大型染色体和5对小型染色体组成,染色体简式为:2n=9m 2sm;中国林蛙的二倍体染色体数目为2n=24,由5对大型染色体和7对小型染色体组成,染色体简式为:2n=5m 5sm 1st 1t。这可用于不同地域同类物种的鉴定和分类。

关 键 词:甘肃  中华蟾蜍  中国林蛙  染色体  核型
文章编号:1671-1351(2007)05-0026-03
修稿时间:2007年4月27日

Preliminary Study on Karyotypes of Bufo gargarizans cantor and Rana chensinensis
Yuan Yijun,Yang Jinyi,Yuan Huijun,Yang Xiaoliang.Preliminary Study on Karyotypes of Bufo gargarizans cantor and Rana chensinensis[J].Journal of Tianshui Normal University,2007,27(5):26-28.
Authors:Yuan Yijun  Yang Jinyi  Yuan Huijun  Yang Xiaoliang
Abstract:After injecting yeast extract and colchicines into Bufo gargarizans cantor and Rana chensinensis of Gansu,the chromosome specimen slice is made from marrow cells and respectively carried on the preliminary analysis to karyotypes of them.The results show that the number of diploid chromosome in Bufo gargarizans cantor is 2n=22,which is composed by six pairs of large chromosome and five pairs of small chromosome,and its karyotype skeleton symbol is 2n=9m 2sm.That of Rana chensinensis is 2n=24,which is composed by five pairs of large chromosome and seven pairs of small chromosome,and its karyotype skeleton symbol is 2n=5m 5sm 1st 1t.The experiment provides some effective cytogenetic data for identification and classifying the coordinal species in different regions.
Keywords:Gansu  Bufo gargarizans cantor  Rana chensinensis  chromosome  karyotype
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