| 补充扇贝寡肽对运动大鼠骨骼肌废用性萎缩的干预研究 |
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| 引用本文： | 郭振新.补充扇贝寡肽对运动大鼠骨骼肌废用性萎缩的干预研究[J].广州体育学院学报,2013,33(3):87-93. |
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| 作者姓名： | 郭振新 |
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| 作者单位： | 广东罗定职业技术学院,广东罗定,527200 |
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| 摘 要： | 目的通过组织学、免疫组化方法和Calpain活性的测试,观察扇贝寡肽对运动大鼠停训致肌萎缩后肌肉湿重、肌细胞形态、肌纤维直径、横截面积、Calpain活性、肌球蛋白表达以及肌细胞凋亡过程的影响,研究扇贝寡肽对停训后骨骼肌萎缩的干预作用.方法40只SD雄性大鼠以15m/min的速度,持续性水平跑台训练30min,运动6d/week,休息1 d/week,其后以速度递增3m/min/week,运动时间递增30min/week,在为期3周的训练后即随机抽取10只大鼠为基础对照组(A,n=10),其余大鼠为B组用于制备骨骼肌萎缩模型,3周后随机分组为补充扇贝寡肽组(B1,n=10)、补充安慰剂组(B2,n=10)、补充牛乳组(B3,n=10).实验组所有动物每天分别进行灌胃15％扇贝寡肽饮料2ml、B3组大鼠灌胃等量的牛乳,B2组大鼠灌胃等量的安慰剂.结果固定大鼠左侧后肢3周后可导致骨骼肌废用性萎缩,废用性肌萎缩的发生、发展与Calpain活性升高、水解骨骼肌收缩蛋白有关,而且,骨骼肌细胞凋亡增加在废用性肌萎缩的发生发展过程中产生重要作用.而补充扇贝寡肽可显著减低骨骼肌细胞凋亡指数,明显抑制骨骼肌Bax蛋白的表达、促进Bcl-2蛋白的表达,抑制Calpain活性,减少收缩蛋白的降解,维持肌球蛋白结构的完整,从而干预肌萎缩的进程.
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| 关 键 词： | 扇贝寡肽 停训 骨骼肌萎缩 细胞凋亡 Calpain 肌球蛋白 大鼠 |
| 修稿时间： | 2013/2/11 0:00:00 |
Study of the Effects of Scallop Peptides Supplementation on Muscular Atrophy after Rats Detraining |
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| GUO Zhen-xing.Study of the Effects of Scallop Peptides Supplementation on Muscular Atrophy after Rats Detraining[J].Journal of Guangzhou Physical Education Institute,2013,33(3):87-93. |
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| Authors: | GUO Zhen-xing |
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| Institution: | (Luoding polytechnic college,Luoding 527200,China) |
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| Abstract: | Objective Through histology and immunochemistry methods and the test of Calpain activity, this paper studies the effects of scallop peptides supplementation on muscle morphology, the Calpain activity, expression of Myosin and muscle cell apoptosis, after detraining. Methods 40 Male Sprague-Dawley rats ran 30 minutes at the speed of 15m/min , exercise 5day/week and rest 1day/week in the first week. In second week, rats ran 60 minutes at the speed of 18m/min, exercise 5day/week and rest 1day/week and ran 90 minutes at the speed of 21m/min, exercise 5day/week and rest 1day/week in the third week. After sports training for 3 weeks, ten rats were baseline group. The 30 rats were divided randomly into 3 groups as placebo, milk and SPs supplemented, then the left hind limbs of the rats were fixed by plastic gypsum for 3 weeks. Rats in groups B3 were fed 2ml beverage containing 15% SPs, and rats in control were fed 2ml placebo and milk respectively. Results The results showed that skeletal muscle were atrophy, after left hind limbs fixed for three weeks. Activated Calpain and hydrolyzed myosin took important roles in the process of muscular atrophy events. After SPs supplementation, the loss of mass for muscle, the Calpain activity alleviated obviously, the integrity of the myosin structure was maintained and the expression for Bcl-2 increased obviously. But the expression for Bax decreased obviously. |
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| Keywords: | scallop peptides detraining muscular atrophy apoptosis Calpain myosin rat |
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