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| Ⅱ型糖尿病大鼠运动后骨骼肌IRS-2、GLUT4含量及IRS-2磷酸化变化的时间特征 | |
| 引用本文： | 付颖,王耀光,李永春.Ⅱ型糖尿病大鼠运动后骨骼肌IRS-2、GLUT4含量及IRS-2磷酸化变化的时间特征[J].首都体育学院学报,2011,23(4):372-375. |
| 作者姓名： | 付颖 王耀光 李永春 |
| 作者单位： | 1. 国家体育总局奥林匹克体育中心,北京,100029 2. 辽宁师范大学体育学院,大连,116029 3. 辽宁警官高等专科学校,大连,116033 |
| 基金项目： | 辽宁省教育厅自然科学基金资助课题(20060474) |
| 摘 要： | 为了解运动后大鼠肌细胞IRS-2、GLUT4蛋白含量及IRS-2磷酸化的动态变化及时间特征。制备II型糖尿病大鼠模型。将SD大鼠分为正常对照组、糖尿病对照组和糖尿病运动组。用Western blot免疫印迹法检测运动后IRS-2、GLUT4蛋白含量及IRS-2磷酸化程度。结果得出:与糖尿病对照组相比,运动后1 h、3 h组、6 h、12 h、24 h和48 h股四头肌IRS-2蛋白含量分别升高6.8%、23.7%(P<0.01)、20.7%(P<0.01)、10%、5.4%和2.7%;GLUT4蛋白含量分别显著升高17.3%、32.7%、42.3%、46.2%、51.9%和45%(P<0.01);IRS-2磷酸化分别升高49%、51.4%、45.1%、43.1%、21.6%(P<0.01)和9.8%(P<0.05)。结论:一次性中等强度的运动对IRS-2、GLUT4含量和IRS-2磷酸化产生显著的影响,其中GLUT4含量和IRS-2磷酸化升高的效应可持续到运动结束的48 h。 |
| 关 键 词： | 运动后时间特征 Ⅱ型糖尿病 大鼠 IRS-2 磷酸化 GLUT4 |
Dynamic Effects of Exercise on Content of IRS-2 and GLUT4 and IRS-2 Tyrosine Phosphorylation in Skeletal Muscle of DM Rats |
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| FU Ying,WANG Yaoguang,LI Yongchun.Dynamic Effects of Exercise on Content of IRS-2 and GLUT4 and IRS-2 Tyrosine Phosphorylation in Skeletal Muscle of DM Rats[J].Journal of Capital College of Physical Education,2011,23(4):372-375. | |
| Authors: | FU Ying WANG Yaoguang LI Yongchun |
| Institution: | 1.National Olympic Sports Centre,Beijing 100029,China;2.College of Physical Education,Liaoning Normal University,Dalian 116029,China;3.Liaoning Advanced Police Officer School,Dalian 116033,China. |
| Abstract: | Objective:To explore the dynamic effects of exercise on protein content of IRS-2,GLUT4 and IRS-2 tyrosine phosphorylation in DM rats.Method:SD rats were divided into normal control group(NC),diabetes mellitus group(DMC) and exercise group.The exercise group did one-bout exercise for 50min.The protein contents of IRS-2,GLUT4 and IRS-2 tyrosine phosphorylation were measured using Western bolt analysis.Result:The contents of IRS-2 protein in 1 h,3 h,6 h,12 h,24 h and 48 h groups increased up to 6.8%,23.7%(P<0.01),20.7%(P<0.01),10%,5.4% and 2.7%;GLUT4 protein in 1 h,3 h,6 h,12 h,24 h and 48 h groups increased up to 17.3%,32.7%,42.3%,46.2%,51.9% and 45%(P<0.01).The tyrosine phosphorylation of IRS-2 protein in 1 h,3 h,6 h,12 h,24 h and 48 h groups increased up to 49%,51.4%,45.1%,43.1%,21.6%(P<0.01) and 9.8%(P<0.05) respectively compare with the DMC group.Conclusion:The results showed that the contents and the tyrosine phosphorylation of IRS-2 and GLUT4 protein have the obviously effect after once bout exercise,and the effect could last for 48 h after exercise. |
| Keywords: | time trait after exercise Ⅱ diabetes rat IRS-2 phosphorylation GLUT 4 |
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