| 运动与IL-6shRNA干扰影响胰岛素抵抗发生的作用及其机制研究 |
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| 引用本文： | 唐晖,雷雨,周亮,蔡建光.运动与IL-6shRNA干扰影响胰岛素抵抗发生的作用及其机制研究[J].成都体育学院学报,2012,38(5):72-78. |
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| 作者姓名： | 唐晖 雷雨 周亮 蔡建光 |
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| 作者单位： | 1. 湖南科技大学体育学院,湖南湘潭,411201
2. 铜仁学院体育系,贵州铜仁,554300 |
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| 基金项目： | 国家自然科学基金面上项目 |
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| 摘 要： | 目的:探讨运动是否通过IL-6抑制胰岛素抵抗的发生,以及p38MAPK信号蛋白在此的可能作用。方法:大鼠高脂饮食8周以形成胰岛素抵抗,通过葡萄糖钳夹实验确定胰岛素抵抗是否发生;在此过程中采用8周跑台运动和/或IL-6shRNA载体注射干预,检测血清IL-6、空腹胰岛素、空腹血糖、葡萄糖输注率、各组织IL-6基因表达水平及磷酸化p38活性等指标。结果:高脂饮食8周形成胰岛素抵抗,IL-6shRNA注射组各组织IL-6mRNA水平与对照组相比显著降低(P<0.05,或P<0.01);8周有氧运动显著降低胰岛素抵抗发生,但是运动注射IL-6shRNA组的胰岛素抵抗与运动不注射注射IL-6shRNA组相比葡萄糖输注率显著降低(P<0.05,或P<0.01),高脂饮食大鼠磷酸化p38显著升高,运动高脂饮食大鼠与之相比显著降低(P<0.05,或P<0.01),但是运动注射IL-6shRNA组大鼠磷酸化p38的变化却没有明显规律。结论:运动抑制胰岛素抵抗的发生可能通过激活IL-6和/或下调p38MAPK信号通路而起作用。
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| 关 键 词： | p38MAPK 运动 IL-6shRNA 胰岛素抵抗 |
The effects and mechanism study on the development of insulin resistance intervened by exercise and IL-6shRNA |
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| Institution: | TANG Hui et al(Hunan University of Science and Technology,Xiangtan Hunan 411201) |
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| Abstract: | Objective: To probe into whether exercise inhibited the development of insulin resistance(IR) through IL-6 and the possible effects of p38MAPK signal pathway.Methods: Rats were fed by 8 weeks high-fat diets to develop IR,and glucose infusion rates(GIR) were determined by hyperinsulinemic-euglycemic clamping to confirm the development of IR.8 weeks treadmill exercise and/or IL-6shRNA plasmid injection were adopted.The indexes of serum IL-6,fasting blood glucose,fasting serum insulin,GIR,IL-6 gene expression levels and p-p38 in various tissues were determined.Results: Rats fed by 8 weeks high-fat diets were developed IR and the IL-6mRNA levels in various tissues of IL-6shRNA injection groups were significantly lower than those of control group(P<0.05,or P<0.01).The development of IR in exercise rats significantly decreased,however,compared with that,the GIR of exercise rats injected by IL-6shRNA was lower(P<0.05,or P<0.01).The p-p38 significantly increased in the rats fed by high-fat diets,however,p-p38 in the exercise high-fat diets rats significantly decreased than that(P<0.05,or P<0.01).The changes of p-p38 in exercise rats injected by IL-6shRNA were irregular.Conclusions: These results suggested that exercise inhibited the development of IR by activating IL-6 and/or inhibiting p38 MAPK signal pathway.The up-regulation of IL-6 gene expression possibly inhibited p38 MAPK signal pathway. |
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| Keywords: | p38MAP exercise IL-6shRNA IR |
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