| 一种可以实现稳定单细胞包裹的无进样器的微流控平台 |
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| 作者姓名： | 范蓓媛 刘力行 李秀锋 陈德勇 王文会 王军波 陈健 |
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| 作者单位： | 1. 中国科学院电子学研究所传感技术国家重点实验室, 北京 100190;
2. 中国科学院大学, 北京 100049;
3. 清华大学精密仪器系, 北京 100084 |
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| 基金项目： | Supported by the National Basic Research Program of China (2014CB744600), National Natural Science Foundation of China (61431019, 61671430), Chinese Academy of Sciences Key Project Targeting Cutting-Edge Scientific Problems (QYZDB-SSW-JSC011), Instrument Development Program of the Chinese Academy of Sciences and Youth Innovation Promotion Association of Chinese Academy of Sciences |
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| 摘 要： | 液滴单细胞包裹技术是实现单细胞研究的有效方法。采用负压作为驱动力包裹单细胞，不再需要常规实验中会引起细胞吸附和降低单细胞包裹率的进样器和管路。在细胞悬浮溶液中加入碘克沙醇溶液调整溶液密度，进一步改善细胞沉降问题。研究负压大小和T型沟道几何尺寸对液滴频率和体积的影响以及碘克沙醇调整溶液密度后对单细胞包裹率的影响。该微流控平台可实现稳定的单细胞包裹，并为单细胞分析提供有利工具。
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| 关 键 词： | 液滴微流控 单细胞包裹 负压 碘克沙醇 |
| 收稿时间： | 2018-10-10 |
| 修稿时间： | 2019-03-04 |
Study of syringe-free droplet microfluidic platforms enabling stable single-cell encapsulation |
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| Authors: | FAN Beiyuan LIU Lixing LI Xiufeng CHEN Deyong WANG Wenhui WANG Junbo CHEN Jian |
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| Institution: | 1. . State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China;
2. . University of Chinese Academy of Sciences, Beijing 100049, China;
3. . Department of Precision Instrument, Tsinghua University, Beijing 100084, China |
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| Abstract: | Negative pressures were used to generate droplets for single-cell encapsulations. Iodixanol was used to change the suspension solution densities in order to reduce the issue of cellular sedimentations. The effects of negative pressures and geometries of microfluidic T-channels on volumes and frequencies of generated droplets and the effects of iodixanol on the percentages of single-cell encapsulations were investigated and compared. This microfluidic platform may enable stable single-cell encapsulations, paving ways for single-cell analysis. |
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| Keywords: | droplet microfluidics single-cell encapsulation negative pressure iodixanol |
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