| 低氧预适应对小鼠骨骼肌Nrf2和线粒体呼吸链复合物蛋白表达及运动能力的影响 |
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| 引用本文： | 王林佳,魏昊,严露,张缨.低氧预适应对小鼠骨骼肌Nrf2和线粒体呼吸链复合物蛋白表达及运动能力的影响[J].北京体育大学学报,2019,42(2):126-132. |
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| 作者姓名： | 王林佳 魏昊 严露 张缨 |
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| 作者单位： | 北京体育大学 北京 100084,北京体育大学 北京 100084,北京体育大学 北京 100084,北京体育大学 北京 100084 |
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| 基金项目： | 国家自然科学基金资助项目（31471134）；北体大国际合作项目（2018GJ007）。通信作者：张缨。 |
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| 摘 要： | 摘要：目的：低氧预适应是指经过短暂的轻度缺氧后，对后续的的严重缺氧产生耐受作用。最新的研究发现核因子E2相关因子2（Nrf2）除参与抗氧化作用外，对线粒体的生物合成和机体的能量代谢也发挥重要的调节作用。本研究试图通过对小鼠进行低氧预适应，探究其对骨骼肌Nrf2及线粒体呼吸链复合物蛋白表达和运动能力的影响。方法：健康8周龄C57BL/6J小鼠（WT）共40只，随机分为四组：未低氧预适应安静组（NC），低氧预适应安静组（HC），未低氧预适应低氧运动组（NE）和低氧预适应低氧运动组（HE），每组10只。对低氧预适应组小鼠进行48h持续低氧暴露，氧浓度为11.2%，未低氧预适应组小鼠饲养于常氧下。低氧预适应组低氧暴露结束后，对运动组小鼠进行运动能力测试。运动后即刻取小鼠腿部胫骨前肌检测Nrf2及线粒体呼吸链复合物的蛋白表达，实时荧光定量PCR检测NRF1、TFAM的基因表达。结果：（1）与未低氧预适应组相比，低氧预适应安静组和运动组Nrf2蛋白表达均显著增加（P<0.05）；（2）安静状态下，低氧预适应小鼠TFAM mRNA表达和线粒体呼吸链复合物CI-CV蛋白表达呈下降趋势。（3）一次性低氧力竭运动后，低氧预适应小鼠NRF1和TFAM mRNA和线粒体呼吸链复合物CI、CIII、CIV、CV蛋白表达出现增加趋势，CII蛋白表达显著下降（P<0.05）。（4）经过低氧预适应的小鼠在低氧环境中的运动能力显著增加，体现为力竭时小鼠跑动距离显著增加和达到力竭时所用时长显著增加。结论：48小时11.2%氧浓度的低氧预适应，可显著增加低氧运动后小鼠骨骼肌Nrf2和线粒体呼吸链复合物CIV和CV蛋白表达，这可能是低氧预适应提高小鼠运动能力的原因之一。
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| 关 键 词： | 关键词：低氧预适应 能量代谢 线粒体呼吸链复合物 运动能力 |
| 收稿时间： | 2018/12/25 0:00:00 |
Effects of hypoxic preconditioning on Nrf2 and mitochondrial respiratory chain complex protein expression and exercise ability in skeletal muscle of mice |
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| WANG Lin-ji,WEI Hao,YAN Lu and ZHANG Ying.Effects of hypoxic preconditioning on Nrf2 and mitochondrial respiratory chain complex protein expression and exercise ability in skeletal muscle of mice[J].Journal of Beijing Sport University,2019,42(2):126-132. |
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| Authors: | WANG Lin-ji WEI Hao YAN Lu and ZHANG Ying |
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| Institution: | Beijing Sport University, Beijing 100084, China,Beijing Sport University, Beijing 100084, China,Beijing Sport University, Beijing 100084, China and Beijing Sport University, Beijing 100084, China |
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| Abstract: | Abstract: Objective: Hypoxic preconditioning refers to the tolerance to subsequent severe hypoxia after a short period of mild hypoxia. Recent studies have found that Nrf2 plays an important regulatory role in mitochondrial biosynthesis and energy metabolism in addition to its antioxidant role. In this study, we tried to explore the influence of hypoxic preconditioning on the exercise capacity and the expression of mitochondrial respiratory chain complex protein expression in mice. Methods: Forty healthy 8-week-old C57BL/6J mice (WT) were randomly divided into four groups (n = 10): normal control (NC), hypoxic preconditioning control (HC), normal exercise (NE) and hypoxic preconditioning and exercise (HE) groups. Mice in the HC and HE groups were exposed to hypoxic for 48h at oxygen concentration of 11.2%, while those in the NC and NE groups were kept under normoxic. After the hypoxia preconditioning, the exercise capacity of mice in the exercise groups was tested. The anterior tibial muscles of mice were taken immediately after exercise, the protein expression of mitochondrial respiratory chain complex was detected, and the gene expressions of NRF1, TFAM were detected by real-time fluorescence quantitative PCR. Results: 1) Compared with NC and NE groups, the Nrf2 protein expression increased in HC and HE groups (P < 0.05). 2) The mRNA expression of TFAM and the protein expression of mitochondrial respiratory chain complex CI-CV in HC mice showed a downward trend. 3) After one-time exhaustive exercise under hypoxic environment, the expression of NRF1 and TFAM and mitochondrial respiratory chain complex CI, CIII, CIV and CV in HE increased, while the expression of CII protein decreased significantly (P < 0.05). 4) The exercise capacity of hypoxic preconditioned mice in hypoxic environment was significantly increased, which was reflected in the significant increase of running distance and the significant increase of time spent in exhaustion. Conclusions: Hypoxic preconditioning with 11.2% oxygen concentration at 48 hours significantly increased the expression of Nrf 2 and mitochondrial respiratory chain complex CIV and CV protein in skeletal muscle of mice after hypoxic exercise, which may be one of the reasons for hypoxic preconditioning to improve the exercise capacity of mice. |
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| Keywords: | Keywords: hypoxic preconditioning energy metabolism mitochondrial respiratory chain complex exercise capacity |
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