| 运动与注射IGF-1对大鼠骨骼肌mTOR、AR蛋白表达的影响 |
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| 引用本文： | 郝鑫鑫,曾凡星,赵华,欧阳芡.运动与注射IGF-1对大鼠骨骼肌mTOR、AR蛋白表达的影响[J].北京体育大学学报,2010(5). |
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| 作者姓名： | 郝鑫鑫 曾凡星 赵华 欧阳芡 |
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| 作者单位： | 北京体育大学;天水师范学院体育学院; |
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| 基金项目： | 国家自然科学基金资助项目(批准号:30671013) |
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| 摘 要： | 目的:观察运动、注射IGF-1对大鼠骨骼肌内IGF-1含量、mTOR、AR蛋白表达的影响,探讨IGF-1对mTOR、AR的调节作用。方法:雄性成年SD大鼠24只,随机分为4组,安静对照组(C)、安静+IGF-1注射组(CI)、运动组(E)、运动+IGF-1注射组(EI),每组6只。运动方案为60 min/d,强度20 m/min,坡度10%,持续一周的跑台训练。每天训练后大鼠小腿后侧皮下注射IGF-I,对照组注射安慰剂。最后一次运动后6 h取胫骨前肌,测定IGF-1含量、mTOR、AR蛋白表达。结果:1)与C组比较,E、EI组骨骼肌内IGF-1含量、mTOR蛋白表达显著升高;2)C、CI、E、EI组骨骼肌内AR蛋白表达呈下降趋势。结论:1周耐力运动可导致肌肉内IGF-1含量、mTOR蛋白表达显著增加,而肌肉中AR蛋白表达却呈现出下降趋势,其原因有待于进一步研究。
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| 关 键 词： | 运动 骨骼肌 胰岛素样生长因子-1 哺乳动物雷帕霉素靶蛋白 雄激素受体 |
The Effect of Insulin-like Growth Factor 1(IGF-1) and Exercise on the Protein Expression of Mammalian Target of Rapamycin(mTOR) and Androgen Receptor(AR) in Rats Skeletal Muscle |
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| HAO Xin-xin,ZENG Fan-xing,ZHAO Hua,OUYANG Qian.The Effect of Insulin-like Growth Factor 1(IGF-1) and Exercise on the Protein Expression of Mammalian Target of Rapamycin(mTOR) and Androgen Receptor(AR) in Rats Skeletal Muscle[J].Journal of Beijing Sport University,2010(5). |
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| Authors: | HAO Xin-xin ZENG Fan-xing ZHAO Hua OUYANG Qian |
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| Institution: | HAO Xin-xin1,ZENG Fan-xing1,ZHAO Hua2,OUYANG Qian1(1.Beijing Sport University,Beijing 100084,China,2.P.E.Department,Tianshui Normal University,Tianshui 741001,Gansu China) |
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| Abstract: | The study examined the effects of IGF-1 injection and exercise on the protein expression of mTOR,AR and IGF-1 level in rats skeletal muscle.24 male Sprague-Dawley rats were randomly divided into four groups: inactive control(C), inactive plus IGF-1 injection(CI),exercise(E),exercise plus IGF-1 injection(EI).After all groups had been accustomed for one week,the Group E & EI performed closely monitored training in the treadmill(grade of 5,at the speed of 20m/min,duration of 1 hour) for 7 days.When the exercis... |
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| Keywords: | exercise skeletal muscle IGF-1 mTOR AR |
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