| 大强度离心运动后热休克蛋白70在骨骼肌细胞中的表达特征研究 |
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| 引用本文： | 高前进,张晓峰.大强度离心运动后热休克蛋白70在骨骼肌细胞中的表达特征研究[J].北京体育大学学报,2010(9). |
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| 作者姓名： | 高前进 张晓峰 |
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| 作者单位： | 石家庄学院体育系;河北司法警官职业学院; |
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| 摘 要： | 目的:观察离心运动后热休克蛋白70在骨骼肌细胞内的表达特征,探讨热休克蛋白70的保护作用机制。方法:成年雄性Wistar大鼠24只,随机分为对照组和运动组,运动组进行一次性大负荷离心运动,于运动后即刻和24 h取腓肠肌,冰冻切片,用腺苷三磷酸酶法和免疫荧光组织化学法观察热休克蛋白70的亚细胞分布特征。结果:安静状态热休克蛋白70分布在Ⅰ,IIa型肌纤维,IIb型肌纤维中没有分布。离心运动后热休克蛋白70从胞浆内移位到Ⅰ,IIa,IIb肌纤维的细胞膜和细胞核。结论:热休克蛋白70作为分子伴侣,在肌纤维遭到应激时移位到细胞膜和核,可能对细胞膜起保护作用,也可能在膜损伤后,移位于此帮助蛋白合成修复。
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| 关 键 词： | 离心运动 热休克蛋白70 肌纤维类型 |
Study on Heat Shock Protein 70 Expression Characteristic of Skeletal Muscle Cell after High-Intensity Eccentric Exercises |
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| GAO Qian-jin,MA Xin-dong.Study on Heat Shock Protein 70 Expression Characteristic of Skeletal Muscle Cell after High-Intensity Eccentric Exercises[J].Journal of Beijing Sport University,2010(9). |
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| Authors: | GAO Qian-jin MA Xin-dong |
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| Institution: | GAO Qian-jin1,MA Xin-dong2(1.Dept.of P.E.Shijiazhuang University,Shijiazhuang 050035,Hebei China,2.Tsinghua University,Beijing 100084,China) |
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| Abstract: | Objective: The purpose of this study was to examine the characteristic expression of hsp70 in skeletal muscle cell after the high-intensity eccentric exercise of rats once at a time.The mechanism protective effects of hsp70 in skeletal muscle are observed.Methods: 24 adult male Wistar rats were divided into control group(n=8) and exercise group.The exercise group did eccentric exercises at high intensity once at a time.After exercise,the rat gastrocnemius muscle was taken into cryo-section.And the hsp70 exp... |
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| Keywords: | eccentric exercise Heat shock protein 70 muscle fiber type |
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