| 外源性IGF-I对大鼠运动骨骼肌mTOR及下游信号的影响 |
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| 引用本文： | 曾凡星,朱晗,赵华,欧阳芡.外源性IGF-I对大鼠运动骨骼肌mTOR及下游信号的影响[J].北京体育大学学报,2011,34(4):47-50. |
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| 作者姓名： | 曾凡星 朱晗 赵华 欧阳芡 |
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| 作者单位： | 北京体育大学;天水师范学院体育学院; |
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| 基金项目： | 国家自然科学基金项目“低氧运动对骨骼肌mTOR信号的影响及机制”(批准号30671013) |
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| 摘 要： | 目的:通过观察一周注射外源性IGF-I和跑台运动对mTOR及其下游信号的影响,以深入探讨IGF-I对运动骨骼肌蛋白合成信号的影响机理。方法:8周龄雄性SD鼠在适应性训练后分为四组:安静组(S)、IGF-I组(SI)、运动组(E)、运动+IGF-I组(EI),每组6只。运动方式为跑台运动(坡度为10%,跑速20 m/min,60min),每天一次,共7 d。外源性IGF-I为小腿后侧肌肉隆起处的皮下注射。用Western Blotting法检测腓肠肌MHC、mTOR(Ser2448)、p70S6K(Thr389)和4EBP1(Thr37/46)的磷酸化表达。结果:在一周后,外源性IGF-I显著促进骨骼肌湿重和MHC的表达。外源性IGF-I和运动均显著促进骨骼肌mTOR(Ser 2448)(P<0.01)、p70S6K(Thr 389)(P<0.01)和4EBP1(Thr 37/46)(P<0.01)的磷酸化表达。外源性IGF-I和运动因素在影响mTOR及其下游信号时存在协同促进效应。结论:1)一周外源性IGF-I注射明显促进骨骼肌蛋白合成,且明显强于运动的促合成效应。2)一周外源性IGF-I注射与运动均能明显促进运动骨骼肌mTOR及其下游信号的活性。
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| 关 键 词： | IGF-I mTOR通路 骨骼肌肥大 运动 |
Effects of Exogenous IGF-I and Exercise on mTOR and Downstreams of mTOR of Skeletal Muscle in Rats |
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| ZENG Fan-xing,ZHU Han,ZHAO Hu,OUYANG Qian.Effects of Exogenous IGF-I and Exercise on mTOR and Downstreams of mTOR of Skeletal Muscle in Rats[J].Journal of Beijing Sport University,2011,34(4):47-50. |
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| Authors: | ZENG Fan-xing ZHU Han ZHAO Hu OUYANG Qian |
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| Institution: | ZENG Fan-xing1,ZHU Han1,ZHAO Hua2,OUYANG Qian1(1.Beijing Sport University,Beijing 100084,China,2.Physical Education Department,Tianshui Normal University,Tianshui 741001,Gansu China) |
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| Abstract: | Objective:The purpose of this study was to examine the effect of mTOR and downstream of mTOR on muscle protein synthesis in resistance treadmill exercise model through injecting exogenous IGF-I in vivo.Methods: Adult male Sprague-Dawley rats(8 weeks old) were randomly divided into 4 groups after adaptive training: sedentary(S),IGF-I group(SI),exercise group(E) and IGF-I plus exercise group(EI).The following treadmill training was applied: 20m/min at 10% slope,60 min;once per day,7 days.Rats were treated dai... |
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| Keywords: | IGF-I mTOR pathway muscle hypertrophy exercise |
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