| 饮食和运动干预对高脂饮食性肥胖大鼠心肌间质胶原纤维的影响 |
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| 引用本文： | 李钢.饮食和运动干预对高脂饮食性肥胖大鼠心肌间质胶原纤维的影响[J].北京体育大学学报,2011,34(3):59-63. |
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| 作者姓名： | 李钢 |
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| 作者单位： | 江苏淮阴师范学院体育系; |
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| 摘 要： | 目的:研究饮食和运动干预对肥胖大鼠心肌间质胶原纤维的影响并对其可能的机制进行探讨。方法:将三周龄SD大鼠随机分为正常组和高脂饮食组,肥胖大鼠再分为肥胖对照组、饮食干预组与运动和饮食联合干预组,第16周时处死。用逆转录聚合酶链式反应(RT-PCR)法测定心肌ACEmRNA表达;羟脯氨酸法测定心肌胶原含量;采用双抗体夹心法ABC—ELISA测定血清PICP、PIHNP;苦味酸—天狼星红胶原染色大鼠心脏切片;免疫组化法测定心肌AT1受体和CTGF的表达。结果:肥胖大鼠存在严重的血脂紊乱,心肌胶原含量显著增加,血清1型前胶原羧基端肽、心肌组织ACEmRNA和AT1R、CTGF蛋白表达增加。饮食干预组血脂紊乱程度减轻,心肌胶原含量、血清1型前胶原羧基端肽、ACEmRNA和AT1R、CTGF表达均有降低、Ⅲ型前胶原氨基端肽表达有所升高;运动和饮食联合干预组体重下降明显,心肌胶原含量、血清1型前胶原羧基端肽、ACEmRNA和AT1R、CTGF表达下降更明显、Ⅲ型前胶原氨基端肽表达升高显著。结论:高脂性肥胖大鼠存在心肌间质胶原纤维化,并与ACEmRNA和AT1R、CTGF、血清1型前胶原羧基端肽表达的改变有关系。单纯性饮食疗法能够减缓心肌纤维化的进展,饮食和运动联合干预能够使心肌纤维化早期良性逆转。
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| 关 键 词： | 干预性研究 肥胖 心肌重构 大鼠 |
Influence and Possible Mechanism Analysis on Myocardial Interstitium Remodeling of High-lipid Diet Rats in Diet and Exercise Intervention |
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| LI Gang.Influence and Possible Mechanism Analysis on Myocardial Interstitium Remodeling of High-lipid Diet Rats in Diet and Exercise Intervention[J].Journal of Beijing Sport University,2011,34(3):59-63. |
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| Authors: | LI Gang |
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| Institution: | LI Gang(Huaiyin Normal University,Huaian 223001,Jiangsu China) |
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| Abstract: | Objective: The purpose of this study is to probe into the influence of diet and exercise training on myocardial interstitium remodeling in high-lipid diet rats and discuss possible mechanism.Methods: SD rats(three weeks old) were divided into the normal group and the high-lipid diet group randomly.Then fat rats were divided into three groups randomly,namely obese control group,diet intervention group,diet combined with exercise intervention groups.The fat rats were sacrificed at the end of 16th week.Myocard... |
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| Keywords: | intervention research fat myocardial remodeling rats |
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