| 尾悬吊大鼠股直肌废用性肌萎缩的相关蛋白分析 |
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| 引用本文： | 任媛媛,周越,田志华,吴金富,朱琳.尾悬吊大鼠股直肌废用性肌萎缩的相关蛋白分析[J].北京体育大学学报,2010(11). |
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| 作者姓名： | 任媛媛 周越 田志华 吴金富 朱琳 |
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| 作者单位： | 北京体育大学;北京市肿瘤防治研究所中心实验室; |
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| 基金项目： | 教育部博士学科点专项科研基金(项目编号:20070043008)的资助 |
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| 摘 要： | 目的:通过双向电泳技术分离骨骼肌蛋白质组份,探讨悬吊后肌萎缩组股直肌与正常骨骼肌的差异蛋白。方法:建立大鼠尾悬吊模型,使大鼠后肢去负荷,两周后取大鼠股直肌进行双向电泳技术分离,使用PDQuest软件对凝胶图像进行差异蛋白组学分析。结果:研究发现在悬吊所致肌萎缩后有23个点发生了明显而稳定的改变,其中下调的蛋白有18个,上调的有5个。结论:悬吊致肌萎缩后,股直肌收缩蛋白、骨架蛋白、运输蛋白含量下调,而与代谢有关的蛋白或酶的表达有升有降,表现了在肌萎缩过程中代谢调节的复杂性。
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| 关 键 词： | 双向电泳 后肢悬吊 肌萎缩 |
Proteome analysis of Atrohy by Hindlimb Suspension in Rat Skeletal Muscle |
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| REN Yuan-yuan,ZHOU Yue,TIAN Zhi-hua,WU Jin-fu,ZHU Lin.Proteome analysis of Atrohy by Hindlimb Suspension in Rat Skeletal Muscle[J].Journal of Beijing Sport University,2010(11). |
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| Authors: | REN Yuan-yuan ZHOU Yue TIAN Zhi-hua WU Jin-fu ZHU Lin |
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| Institution: | REN Yuan-yuan1,ZHOU Yue1,TIAN Zhi-hua2,WU Jin-fu1,ZHU Lin1(1.Beijing Sport University,Beijing 100084,China,2.Central Laboratory,Beijing Institute of Cancer Research,Beijing 100142,China) |
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| Abstract: | Objective: To identify major muscle proteins responding significantly to the unloading,Compared with unsuspended controls,we examined protein expression in the rat muscle during 2-week hindlimb suspension by 2-DE.Methods: Establish the model of hindlimb suspension,to make rat unloading,then examined the proteins by 2-DE after 2-week.Result: we found 23 different expression proteins during unloading.Among these,we observed 18 decreased,and 5 increased.Conclusions: After 2-week hindlimb suspension,These resul... |
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| Keywords: | two-dimensional electrophoresis hindlimb suspension skeletal muscle atrophy |
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