| PI3K阻断剂对大鼠运动骨骼肌Akt/mTOR信号的影响 |
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| 引用本文： | 曾凡星,朱晗,赵华.PI3K阻断剂对大鼠运动骨骼肌Akt/mTOR信号的影响[J].北京体育大学学报,2010(11). |
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| 作者姓名： | 曾凡星 朱晗 赵华 |
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| 作者单位： | 北京体育大学;天水师范学院体育学院; |
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| 基金项目： | 国家自然科学基金项目(批准号30671013) |
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| 摘 要： | 目的:通过阻断PI3K信号,以深入探讨Akt/mTOR通路在抗阻运动中骨骼肌蛋白合成的作用。方法:8周龄雄性SD大鼠在适应性训练后分为4组:安静组(S)、阻断剂组(SL)、运动组(E)、运动+阻断剂组(EL),每组6只。运动方式为跑台运动(坡度为10%,跑速20 m/min,60 min),每天1次,共7 d。腹腔注射外源性LY294002(剂量为5.5 mg/kg)。用Western Blotting法检测腓肠肌MHC、Akt和mTOR蛋白表达、Akt(Ser473)和mTOR(Ser2448)磷酸化表达。结果:在一周后,LY294002明显抑制MHC,运动有促进的趋势,并减弱LY294002对MHC的抑制效应。LY294002显著抑制Akt和mTOR蛋白表达、Akt(Ser473)和mTOR(Ser2448)的磷酸化表达,而运动明显增强mTOR、Akt(Ser473)和mTOR(Ser2448)表达。结论:1)阻断PI3K信号可使运动骨骼肌Akt/mTOR通路受到明显抑制,而同时骨骼肌MHC明显降低,明显抑制肌肉蛋白合成。2)运动明显促进该通路的表达,并减弱PI3K阻断剂对该通路的抑制效应。3)PI3K阻...
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| 关 键 词： | LY294002 PI3K Akt/mTOR通路 肌肉肥大 运动 |
Effects of Blocking PI3K and Exercise on Akt/mTOR Pathway of Skeletal Muscle in Rats |
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| ZENG Fan-xing,ZHU Han,ZHAO Hua.Effects of Blocking PI3K and Exercise on Akt/mTOR Pathway of Skeletal Muscle in Rats[J].Journal of Beijing Sport University,2010(11). |
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| Authors: | ZENG Fan-xing ZHU Han ZHAO Hua |
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| Institution: | ZENG Fan-xing1,ZHU Han1,ZHAO Hua2(1.Beijing Sport University,Beijing 100084,China,2.Physical Education Department,Tianshui Normal University,Tianshui 741001,Gansu China) |
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| Abstract: | Objective: The purpose of this study was to examine the effect of Akt/mTOR pathway on muscle protein synthesis in resistance exercise model through blocking PI3K.Methods: Adult male Sprague-Dawley rats(8 weeks old) were randomly divided into 4 groups after adaptive training: sedentary(S),LY294002 group(SL),exercise group(E) and LY294002 plus exercise group(EL).The following treadmill training was applied: 20m/min at 10% slope,60 min;once per day,7 days. Either 8% ethanol or LY294002(5.5mg/kg,diluted with 8%... |
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| Keywords: | LY294002 PI3K Akt/mTOR pathway muscle hypertrophy exercise |
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