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| 运动对大鼠骨骼肌CaMKⅡ磷酸化水平及核内PGC-1、HDAC5蛋白含量的影响 | |
| 引用本文： | 张红学,郭海峰,马延超.运动对大鼠骨骼肌CaMKⅡ磷酸化水平及核内PGC-1、HDAC5蛋白含量的影响[J].天津体育学院学报,2012,27(3):270-274. |
| 作者姓名： | 张红学 郭海峰 马延超 |
| 作者单位： | 1. 郑州大学体育学院,河南郑州,450044 2. 河南科技大学体育学院,河南洛阳,453007 3. 洛阳师范学院体育学院,河南洛阳,471022 |
| 基金项目： | 河南省科技厅科技发展项目（项目编号：122300410257）；河南省科技厅科技计划基金项目（项目编号：120400450006） |
| 摘 要： | 目的:研究运动后不同时间大鼠骨骼肌CaMKⅡ磷酸化水平及核内PGC-1、HDAC5蛋白含量的变化,以探讨运动后PGC -1α基因表达时相性变化的调节机制.方法:9周龄的雄性Wistar大鼠(167±4) g48只,随机分成安静对照组(C)和跑台运动后0h、3h、6h、12 h、24h取材组,共6组,每组8只.大鼠进行0坡度的1h跑台运动,跑台速度34 m/min.Western blot法测定蛋白含量及磷酸化水平,RT-PCR法测定基因表达.结果:PGC-1α mRNA含量,运动后即刻变化不显著,运动后3h、6h显著增加,12h虽仍高于对照组,但与6h相比显著降低,24h恢复至安静水平.核内PGC-1 α蛋白含量运动后各取材点均无显著变化.核内HDAC5蛋白含量则在运动后3h、6h下降显著,12h虽仍低于对照组,但与6h相比显著增加,24 h恢复到安静组水平.CaMKⅡ磷酸化水平,运动后0h,3h显著升高,6h虽仍高于对照组,但与3h相比显著降低,12h恢复至安静水平.结论:运动后PGC-1α基因表达的时相性变化可能是由对应时相变化规律的CaMKⅡ通过调节核内HDAC5含量,解除了转录因子对PGC-1α基因转录的抑制而实现. |
| 关 键 词： | 过氧化物酶体增生物受体γ共激活因子-1α 钙调素依赖蛋白激酶Ⅱ 组蛋白脱乙酰基酶5 |
| 收稿时间： | 1/3/2012 12:00:00 AM |
Effects of Exercise on CaMKⅡ Phosphorylation and Nuclear PGC-1α/HDAC5 Protein Expression in Rats Skeletal Muscle |
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| ZHANG Hongxue , GUO Hangfeng , MA Yanchao.Effects of Exercise on CaMKⅡ Phosphorylation and Nuclear PGC-1α/HDAC5 Protein Expression in Rats Skeletal Muscle[J].Journal of Tianjin Institute of Physical Education,2012,27(3):270-274. | |
| Authors: | ZHANG Hongxue GUO Hangfeng MA Yanchao |
| Institution: | 1.School of PE,Zhengzhou University,Zhengzhou 450044,China;2.School of PE,Henan Scientific and Technical University,Luoyang 471003,China;3.School of PE,Luoyang Normal University,Luoyang 471022,China) |
| Abstract: | Objective: The phosphorylation activity of CaMKⅡ,nuclear PGC-1α and HDAC5 protein content was measured to explore the mechanism of the phasic change of PGC-1α gene expression after treadmill running.Methods: 48 nine week-old male Wister rats(167±4g) were randomly assigned to the Control group and five different groups that were measured at 0h,3h,6h,12h and 24h after exercise.Rats undertook treadmill running for 1 hour at 0° slope.Results: Compared to the Control group,the expression of PGC-1α mRNA was greatly increased 3h and 6h after exercise.Though the abundance of PGC-1α mRNA decreased 12h after exercise,it’s still higher than that in control group and it returns to normal at 24h after exercise.The expression of nuclear PGC-1α protein was unchanged after exercise.The expression of nuclear HDAC5 significantly decreased 3h and 6h after exercise,the 12h group increased greatly compared to the 6h group,though it’s still lower than the control group and it returns to normal at 24h after exercise.Compared to the control group,the phosphorylation activity of CaMKⅡ was greatly increased 0h and 3h after exercise.Though the phosphorylation activity of CaMKⅡ decreased 6h after exercise,it’s still higher than the control group and it returns to normal at 12h after exercise.Conclusion: Phasic change of the expression of PGC-1αmRNA in rat skeletal muscle was observed in this study,and this is possibly induced by CaMKⅡ which lead the HDAC5 out of the nucleus. |
| Keywords: | PGC-1α CaMKⅡ HDAC5 |
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