| 长期耐力训练对大鼠心肌和骨骼肌mTOR信号通路的影响 |
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| 引用本文： | 吴薇,李欣.长期耐力训练对大鼠心肌和骨骼肌mTOR信号通路的影响[J].西安体育学院学报,2010,27(1). |
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| 作者姓名： | 吴薇 李欣 |
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| 作者单位： | 1. 绵阳师范学院,体育与健康教育学院,四川,绵阳,621000
2. 绵阳师范学院,体育与健康教育学院,四川,绵阳,621000;华东师范大学,体育与健康学院,上海,200241 |
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| 摘 要： | 目的 探讨长期耐力训练对大鼠心肌和骨骼肌mTOR信号通路的影响.方法 雄性SD大鼠鼠随机分为长期耐力训练组(n=12)和对照组(n=12),长期耐力训练组从3月龄时开始进行4个月的耐力训练,建立长期耐力训练模型,使用western Blot蛋白印迹技术对大鼠心肌和骨骼肌mTOR信号通路中mTOR蛋白的表达进行测定;使用实时荧光定量PCR技术,对p70S6K和eIF-4E基因mRNA的表达进行测定.结果 长期耐力训练后,大鼠心脏重量和心系数与对照组相比显著增加(P<0.0S)但没有产生病理性·心肌肥大,而腓肠肌的质量与对照组相比没有明显变化;长期耐力训练后,大鼠心肌mTOR蛋白表达,p70S6K和eIF-4E mRNA的表达明显高于对照组(P<0.05),而腓肠肌mTOR蛋白表达,p70S6K和eIF-4E mRNA的表达与对照组相比无显著性变化.结论 长期耐力训练可以增强大鼠心肌mTOR信号通路的表达,产生运动性心肌肥大,改善衰老小鼠的心脏功能;长期耐力训练可通过对大鼠骨骼肌mTOR信号通路表达的影响,抑制骨骼肌的肥大.
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| 关 键 词： | 长期耐力训练 心肌 骨骼肌 |
Effect of Long-term Endurance Exercise Training on mTOR Signaling Pathway in Cardiac Muscle and Skeletal Muscle |
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| WU Wei,LI Xin.Effect of Long-term Endurance Exercise Training on mTOR Signaling Pathway in Cardiac Muscle and Skeletal Muscle[J].Journal of Xi'an Institute of Physical Education,2010,27(1). |
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| Authors: | WU Wei LI Xin |
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| Institution: | WU Wei1,LI Xin1,2(1.Institute of Sports , Healthy Education,Mianyang Normal University,Mianyang 621000,China,2.School of Sports , Health Care,East China Normal University,Shanghai 200241,China) |
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| Abstract: | Objective: To study the effect of long-term endurance training on mTOR Signaling Pathway in cardiac muscle and skeletal muscle.Methods: Male SD rats were randomly divided into control group(n=12) and long-term endurance training group(n=12).After inducing the animal model of long-term endurance training,using the western blotting method to analysis the expression of mTOR in cardiac muscle and gastronemiuse,using the Real-Tim PCR system to analysis the expression of p70S6K and eIF-4E in cardiac muscle and ga... |
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| Keywords: | mTOR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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