| 耐力训练时脂肪甘油三酯脂肪酶与工作肌糖代谢和线粒体密度——基于ATGL基因敲除小鼠的研究 |
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| 引用本文： | 宋刚,张国栋,雷小灿,彭莉,李莉,王雪冰.耐力训练时脂肪甘油三酯脂肪酶与工作肌糖代谢和线粒体密度——基于ATGL基因敲除小鼠的研究[J].武汉体育学院学报,2017,51(10):68-75. |
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| 作者姓名： | 宋刚 张国栋 雷小灿 彭莉 李莉 王雪冰 |
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| 作者单位： | 1. 西南大学 体育学院, 重庆 400715;2. 国家体育总局 体质评价与运动机能监控重点实验室, 重庆 400715;3. 广西大学 体育学院, 广西 南宁 530004;4. 遵义医学院 基础医学院组织与胚胎学教研室, 贵州 遵义 563003 |
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| 基金项目： | 国家自然科学基金资助项目（31360254）；中央高校基本科研业务费专项资金资助项目（SWU1609025，SWU1709116）。 |
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| 摘 要： | 目的：（1）观察ATGL基因敲除小鼠耐力训练时胰岛素抵抗、工作肌糖转运蛋白及线粒体密度的变化；（2）测定Akt、PKC、PPAR-α蛋白激活及含量，探究其与胰岛素抵抗、工作肌糖转运蛋白及线粒体密度的变化之间的关系。方法：ATGL-/-、ATGL+/-和C57BL/6J小鼠跑台耐力训练训练7天，测定小鼠体重、肌糖原、血糖、血胰岛素和胰岛素抵抗，蛋白印记法测定腓肠肌和比目鱼肌GLUT-4（内外膜）、p-Akt、Akt、p-PKC、PKC含量和PPAR-α水平。结果：（1）运动训练后，腓肠肌和比目鱼肌糖原含量、HOMA-IR指数存在基因类型、运动训练的差异（P<0.05）。运动训练引起血糖和血胰岛素在基因类型的差异（P<0.05）。（2）运动训练后，腓肠肌和比目鱼肌Akt、PKC、磷酸化水平、腓肠肌总Akt、PPAR-α蛋白表达存在基因类型、运动训练的差异（P<0.05）。比目鱼肌总Akt表达、腓肠肌和比目鱼肌总PKC、PPAR-α蛋白运动训练的差异不具有显著性意义（P>0.05）。结论：耐力训练时，ATGL通过上调Akt和PKC促进胰岛素敏感性和改善胰岛素抵抗；ATGL上调PPAR-α起到促进线粒体含量。
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| 关 键 词： | 运动生物化学 脂肪甘油三酯脂肪酶 ATGL基因敲除小鼠 肌糖代谢 线粒体密度 跑台训练 胰岛素抵抗 耐力训练 |
Adipose Triglyceride Lipase and Muscle Glycogen Metabolism,Mitochondrial Density and SignalMolecules during Endurance Training -A Study Based on ATGL Knockout Mice |
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| SONG Gang,ZHANG Guodong,LEI Xiaocan,et al.Adipose Triglyceride Lipase and Muscle Glycogen Metabolism,Mitochondrial Density and SignalMolecules during Endurance Training -A Study Based on ATGL Knockout Mice[J].Journal of Wuhan Institute of Physical Education,2017,51(10):68-75. |
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| Authors: | SONG Gang ZHANG Guodong LEI Xiaocan |
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| Institution: | 1. College of P. E., Southwest Univ., Chongqing 400715, China;2. Key Lab of Physical Fitness Evaluation and Sports Function Monitoring, State Sports General Administration, Chongqing 400715, China;3. College of Physical Education, Guangxi Univ., Nanning 530004;4. Dept. of Histology and Embryology, College of Basic Medicine, Zunyi Medical College, Zunyi 563003, China |
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| Abstract: | This study observed the effects of endurance training on insulin resistance and changes in glucose transporter-4 protein, mitochondrial density in ATGL knockout mice; and determination of Akt, PKC, PPAR-α activation and protein content in order to explore the relationship between the changes of insulin resistance, glucose transporter-4 and mitochondrial content. ATGL-/-, ATGL+/-and C57BL/6J mice ran treadmill exercise for seven days, and their muscle glycogen, blood glucose, insulin and insulin resistance were detected and protein western blot GLUT-4,p-Akt, Akt, p-PKC, PKC and PPAR-α levels in gastrocnemius muscle and soleus muscle were determined. It was shown that after exercise training, the glycogen content and HOMA-IR index of gastrocnemius muscle and soleus muscle were different in genotype and exercise training (P<0.05). Differences appeared in blood glucose and blood insulin in exercise training (P<0.05). Moreover, after exercise training, the activation of p-Akt,p-PKC, and the expression of Akt and PPAR-α protein in gastrocnemius muscle and soleus muscle were significantly different in genotype and exercise training (P<0.05). There was no significant difference of the expression of Akt, the total PKC and PPAR-α protein in gastrocnemius and soleus muscle and soleus muscle (P >0.05). It could be concluded that ATGL promoted insulin sensitivity and insulin resistance by increasing Akt and PKC during endurance training, and ATGL promoted the content of mitochondrial by PPAR-α. |
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| Keywords: | adipose triglyceride lipase ATGL knockout mice inositol metabolism mitochondrial density signal molecule treadmill exercise insulin resistance |
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