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Myosin heavy chain isoform composition in the deltoid and vastus lateralis muscles of elite handball players
Authors:Athanasios Mandroukas  Thomas I Metaxas  Zacharoula Papadopoulou  Jan Heller  Nikos V Margaritelis  Kosmas Christoulas
Institution:1. Faculty of Physical Education and Sport, Charles University , Prague, Czech Republic;2. Laboratory of Evaluation of Human Biological Performance, Department of Physical Education and Sports Science, Aristotle University of Thessaloniki , Thessaloniki, Greece;3. Laboratory of Evaluation of Human Biological Performance, Department of Physical Education and Sports Science, Aristotle University of Thessaloniki , Thessaloniki, Greece;4. School of Physical Education and Sport Science, Department of Competitive Sports, Division of Team Handball, Aristotle University of Thessaloniki , Thessaloniki, Greece;5. Laboratory of Exercise Physiology &6. Biochemistry, Department of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki , Thessaloniki, Greece;7. Biochemistry, Department of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki , Thessaloniki, Greece ORCID Iconhttps://orcid.org/0000-0001-5119-427X
Abstract:ABSTRACT

The purpose of the present study was to compare the myosin heavy chain (MHC) isoform composition of the deltoid and vastus lateralis muscles of the dominant and non-dominant limbs in handball players. Eleven male Greek elite handball players (age 22.6 ± 1.9 yrs, training experience 10.6 ± 2.1 yrs, height 184.1 ± 4.1 cm, and weight 81.0 ± 12.5 kg) participated in the study. Four muscle biopsies were obtained from the dominant and non-dominant deltoid and vastus lateralis muscles during the in-season period. The MHC composition was determined using SDS-PAGE. No significant difference was found between the dominant and non-dominant muscles; Deltoid muscle: MHC I (95%CI = ?1.22, 0.33), P = 0.228], MHC ΙΙa (95%CI = ?0.32, 1.59), P = 0.168] and MHC IIx (95%CI = ?1.49, 1.10), P = 0.749]; Vastus lateralis muscle: MHC I (95%CI = ?0.38, 0.63), P = 0.586], MHC ΙΙa (95%CI = ?0.50, 0.65), P = 0.783] and MHC IIx (95%CI = ?1.08, 0.42), P = 0.355]. The findings of the present study indicate that the greater use of the dominant limbs for throwing actions and body movements in handball do not lead to altered MHC isoform composition compared to the non-dominant limbs.
Keywords:Deltoid  handball  muscle biopsies  myosin  vastus lateralis
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