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| 不同负荷运动对大鼠Th1/Th2平衡及脾淋巴细胞PKC活性的影响 | |
| 引用本文： | 苏利强,长利明,张玮,赵广高,郑里翔.不同负荷运动对大鼠Th1/Th2平衡及脾淋巴细胞PKC活性的影响[J].沈阳体育学院学报,2011,30(5):64-68. |
| 作者姓名： | 苏利强 长利明 张玮 赵广高 郑里翔 |
| 作者单位： | 1. 江西中医学院体育教学部,江西南昌,330004 2. 四川省运动技术学院,四川成都,610043 3. 南昌大学教育学院,江西南昌,330031 4. 江西中医学院基础医学院,江西南昌,330004 |
| 基金项目： | 江西省教育厅科学技术研究项目(项目编号为GJJ09284) |
| 摘 要： | 目的:探讨运动中淋巴细胞PKC活性对大鼠Th1/Th2平衡的影响。方法:随机将48只大鼠分成不同运动负荷组及对照组,完成相应负荷运动后采集外周血和脾脏,采用ELISA法测试血清IFN-γI、L-4含量,分离并裂解脾脏淋巴细胞获得上清液,采用BCA法定蛋白浓度,通过ELISA分析P-PKC的含量。结果:一次中等负荷运动对Thl/Th2平衡影响不大,一次负重力竭运动后即刻会使IFN-γ和IL-4均下调,超负荷运动10天则使IFN-γ在8h的恢复期内得不到恢复,使Thl/Th2平衡向Th2方向漂移。脾脏淋巴细胞PKC的活性在一次中等负荷运动过程中、后变化不明显,一次负重力竭运动使其活性下降,8h后即可恢复,但经过10天的超负荷运动后恢复8h的PKC活性仍处于较低水平。结论:中等负荷运动对Thl/Th2平衡和PKC活性影响不大1,0天超负荷运动可以使Thl/Th2平衡向Th2方向漂移,可能是脾淋巴细胞PKC活性下降所致。 |
| 关 键 词： | 运动负荷 干扰素γ 白细胞介素4 蛋白激酶C |
Effects of Different Load Exercises on Th1/Th2 Balances and Activity of PKC in Splenic Lymphocyte of Rats |
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| SU Liqiang,ZHANG Liming,ZHANG Wei,ZHAO Guanggao,ZHENG Lixiang.Effects of Different Load Exercises on Th1/Th2 Balances and Activity of PKC in Splenic Lymphocyte of Rats[J].Journal of Shenyang Sport University,2011,30(5):64-68. | |
| Authors: | SU Liqiang ZHANG Liming ZHANG Wei ZHAO Guanggao ZHENG Lixiang |
| Institution: | SU Liqiang1,ZHANG Liming2,ZHANG Wei1,ZHAO Guanggao3,ZHENG Lixiang4(1.Department of Physical Education,Jiangxi University of Traditional Chinese Medicine,Jiangxi 330004,Nanchang,China,2.Sichuan Sports Skill Institute,Sichuan 610043,Chengdu,3.Physical Education Department of Nanchang University,Jiangxi 330031,4.Basic Medical Science of Jiangxi University of Traditional Chinese Medicine School,China) |
| Abstract: | To explore the effect of different load exercises on Th1/Th2 balances and the activity of PKC in splenic lymphocyte of rats.48 rats were randomly separated into different load exercises group and control group respectively.The authors collected peripheral blood and Spleen after they finished load exercises accordingly,tested the contents of IFN-γ and IL-4 of serum by ELISA,split spleen and lymph to get clear supernatant liquid,tested the density of albumen using BCA and analyzed the contents of P-PKC through ELISA.Results show that one moderately load exercise doesn’t have great effect on Thl/Th2 balances,IFN-γ and IL-4 are down at the immediate postexercise after weight loading exercises,and IFN-γ can’t be recovered in 8h with overload for 10 days,which cause the drift from Thl/Th2 balances to Th2.It’s not obvious for the changes of the activity of PKC in splenic lymphocyte,while the activity is lower after a weight loading exhausting exercise and it can be recovered in 8h.However,the activity of PKC is at low level after 10 days overload exercises and recovery in 8h.The conclusion is that a moderately load exercise doesn’t have great effect on Thl/Th2 balance and the activity of PKC.However,overloading for 10 days makes it drift from Thl/Th2 balance to Th2,which may be caused by the much lower activity PKC in splenic lymphocyte. |
| Keywords: | exercise load interferon gamma interleukin 4 protien kinase C |
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