| 耐力训练对急性酒精性肝损伤线粒体自噬功能的影响及机制研究 |
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| 引用本文： | 马国栋,刘艳环.耐力训练对急性酒精性肝损伤线粒体自噬功能的影响及机制研究[J].体育科学,2011,31(10):85-90. |
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| 作者姓名： | 马国栋 刘艳环 |
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| 作者单位： | 山东理工大学体育学院,山东淄博,255049 |
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| 摘 要： | 目的:研究耐力训练对大鼠急性酒精性肝损伤肝脏线粒体自噬变化规律及其机制。方法:以SD大鼠建立急性酒精性肝损伤模型,以12周无负重游泳为运动手段,测定血清ALT和AST以及肝脏线粒体活性氧生成、线粒体MDA含量、线粒体顺乌头酸酶活性、线粒体膜电位、线粒体自噬蛋白BNIP3、NIX和HIF-1αmRNA表达以及HIF-1α蛋白表达。结果:急性酒精摄入导致血清ALT和AST显著升高以及肝脏线粒体活性氧生成显著升高,线粒体MDA含量显著升高,线粒体顺乌头酸酶活性显著降低,线粒体膜电位显著降低,线粒体自噬蛋白BNIP3、NIX和HIF-1αmRNA以及HIF-1α蛋白表达显著升高;耐力训练后急性酒精摄入使血清ALT和AST以及肝脏线粒体活性氧生成,线粒体MDA含量,线粒体顺乌头酸酶活性,线粒体膜电位,线粒体自噬蛋白BNIP3、NIX和HIF-1αmRNA以及HIF-1α蛋白表达均表现出与未耐力训练急性酒精摄入相同的变化规律,但变化幅度相对较小;注射HIF-1抑制剂YC-1无论是未耐力训练还是耐力训练大鼠再给予急性酒精摄入时BNIP3、NIX mRNA表达均显著低于未注射抑制剂组,而HIF-1αmRNA表达未见显著性差异,但HIF-1α蛋白表达显著降低。结论:急性酒精摄入引起肝脏线粒体自噬加强,其自噬与HIF-1表达增加有关,而耐力训练可能通过增强肝脏氧气供应能力,进而引起HIF-1表达降低,导致线粒体自噬功能减弱。
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| 关 键 词： | 耐力训练 急性酒精性肝损伤 线粒体自噬 鼠 动物实验 |
The Influence of Endurance Training on the Mitophagy and Its Mechanism in Alcohol-induced Acute Hepatic Injury in Rats |
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| MA Guo-dong,LIU Yan-huan.The Influence of Endurance Training on the Mitophagy and Its Mechanism in Alcohol-induced Acute Hepatic Injury in Rats[J].China Sport Science,2011,31(10):85-90. |
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| Authors: | MA Guo-dong LIU Yan-huan |
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| Institution: | MA Guo-dong,LIU Yan-huan School of Physical Education,Shandong University of Technology,Zibo 255049,China. |
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| Abstract: | Objective:To investigate the change of endurance training on mitophagy and its mechanism in alcohol-induced acute hepatic injury rats.Methods:It analyzed the expressions of BNIP3,NIX and HIF-1α mRNA and content of HIF-1α protein,mitochondrial reactive oxygen species production,content of mitochondrial MDA,activity of aconitase,and mitochondrial potential in liver tissue and ALT and AST in serum in alcohol-induced acute hepatic injury rats after 12 week unload swimming training.Results:The acute alcohol treatment induces mitochondrial ROS production,increases mitochondrial MDA content,decreases aconitase activity and mitochondrial potential,up-regulates expressions of BNIP3,NIX and HIF-1α mRNA and content of HIF-1α protein in liver and increases ALT and AST in serum in alcohol-induced acute hepatic injury rats.The parameters of all above indicate the same change in alcohol-induced acute hepatic injury rats after endurance training but the extent is lower.When rats are injected YC-1(inhibitor of HIF-1),the expressions of BNIP3 and NIX mRNA are lower than that of no injection of YC-1 in trained and untrained rats,however,there is no change of the expression of HIF-1α mRNA but HIF-1α protein increases significantly.Conclusion:Acute alcohol treatment enhances mitophagy,which related to HIF-1 up-regulated expression,but endurance training can increase liver oxygen supply,and that decreases HIF-1expression,which results in the decrease of mitophagy in liver. |
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| Keywords: | endurance training alcohol-induced acute hepatic injury mitophagy rat animal experiment |
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