| 荧光标记法检测活细胞内游离钙离子浓度的改进 |
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| 引用本文： | 许鑫华,傅英松,韩波,何淑兰,王遵,陈强.荧光标记法检测活细胞内游离钙离子浓度的改进[J].科技通报,2003,19(4):282-284. |
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| 作者姓名： | 许鑫华 傅英松 韩波 何淑兰 王遵 陈强 |
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| 作者单位： | 1. 天津大学材料科学与工程学院,天津,300072
2. 南开大学生命科学院,天津,300071 |
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| 基金项目： | 教育部科学技术研究重点项目和天津市自然科学基金(0236091111)资助项目 |
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| 摘 要： | 改进了活细胞内游离钙离子浓度的测定方法，即用惰性基体纳米微球包埋荧光染料，将荧光纳米微球转运进细胞，用于细胞内游离钙离子浓度的实时测定。该方法与传统方法相比，染料不易泄漏，不会出现分室现象，不易光漂白，是一种非常有实用价值的测量活细胞内游离钙离子浓度的方法。
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| 关 键 词： | 分析化学 钙离子 检测 荧光纳米微球 |
| 文章编号： | 1001-7119(2003)04-0282-03 |
| 修稿时间： | 2002年6月11日 |
Improvement on the Detection of [Ca2+]i in Living Cells by Fluorescent Labeling |
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| XU Xin Hua,FU Ying song,HAN Bo,HE Shu lan,WANG Zun,CHEN Qiang.Improvement on the Detection of [Ca2+]i in Living Cells by Fluorescent Labeling[J].Bulletin of Science and Technology,2003,19(4):282-284. |
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| Authors: | XU Xin Hua FU Ying song HAN Bo HE Shu lan WANG Zun CHEN Qiang |
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| Institution: | XU Xin Hua 1 FU Ying song 1 HAN Bo 1 HE Shu lan 1 WANG Zun 1 CHEN Qiang 2 |
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| Abstract: | The fluorescent labeling method to detect Ca 2+ ] i in living cells was improved. The fluorescent indicator was embedded in inert nano sphere matrix, then nano spheres were delivered into cells for real time measurement of Ca 2+ ] i in living cells. Compared with traditional methods, the new one reduced the leach of fluorescent indicator, avoided sequestration of fluorescent indicator and decreased the possibility of photobleaching. The modified method is valuable for detecting Ca 2+ ] i in living cells. |
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| Keywords: | analytical chemistry calcium ion detection fluorescent nano sphere |
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