| 利用RAPD分子标记评价仲彬草属的种间关系 |
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| 引用本文： | 周永红,郑有良,杨俊良,颜济,贾继增.利用RAPD分子标记评价仲彬草属的种间关系[J].中国科学院研究生院学报,2000,38(6):515-521. |
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| 作者姓名： | 周永红 郑有良 杨俊良 颜济 贾继增 |
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| 摘 要： | 利用随机扩增多态性DNA(RAPD)技术分析了14种仲彬草属Kengyilia植物的种间关系。对34个OPRON公司十聚体随机引物进行多态性筛选，20个(58．8％)能产生多态性。14个引物产生的112个DNA片断，用于计算种间Jaccard遗传相似性系数分析，在NTSYS程序中利用UPGMA构建系统发育树状图。分析结果表明：(1)14个Kengyilia物种存在较大的遗传多样性；(2)青藏高原的物种与新疆的物种 的RAPD变异极大；(3)形态相似、地理分布一致的物种有一定的亲缘关系，聚类在一起；(4)RAPD结果与形态学和细胞学等分析结果一致。RAPD分析方法将为Kengyilia系统分类提供DNA水平上丰富的资料。
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| 关 键 词： | 仲彬草属 RAPD分析 遗传变异 亲缘关系 形态学 |
Relationships among Kengyilia species assessed by RAPD markers |
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| ZHOU Yong-Hong,ZHENG You-Liang,YANG Jun-Liang,YEN Chi,JIA Ji-Zeng.Relationships among Kengyilia species assessed by RAPD markers[J].Journal of the Graduate School of the Chinese Academy of Sciences,2000,38(6):515-521. |
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| Authors: | ZHOU Yong-Hong ZHENG You-Liang YANG Jun-Liang YEN Chi JIA Ji-Zeng |
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| Abstract: | To assess the interspecific relationships in Kengyilia, fourteen species of Kengyilia were
used for RAPD assay. Of the 34 random primers tested, twenty (58.8%) produced polymorphic
products. A total of 112 products amplified from 14 primers were selected for RAPD analysis.
Eighty-three (74.1%) amplified products were found to be polymorphic among 14 species. Data
were used to generate Jaccard's similarity coefficients and to construct a dendrogram using UPGMA
in the NTSYS computer programs. It is concluded from this study that: (1) There was great genetic
diversity among the 14 species of Kengyilia; (2) Distinct RAPD variations existed between the spe-
cies from the Qinghai-Xizang Plateau and those from Xinjiang; (3) The species with similar morpho-
logical characters and the species from the same areas or neighboring geographical regions were clus-
tered together; (4) RAPD results are comparable with those obtained from studies on morphology
and cytology. RAPD assay is a useful additional method for assessing the relationships among thespecies in Kengyilia. |
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| Keywords: | Kengyilia RAPD assay Genetic variation Relationships Morphology |
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