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Poly A RNA status and expression of milli (m) and micro (μ) calpains in skeletal muscle of duchenne muscular dystrophy patients |
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| Authors: | Harleen Mangat Tajamul Hussain C Sundaram MP JS Anandaraj |
| Institution: | (1) Present address: Centre for DNA fingerprinting & Diagnostics, Nacharam, Hyderabad, India;(2) Department of Pathology, Nizams Institute of Medical Sciences, Punjagutta, Hyderabad, India;(3) Department of Molecular Biology & Biochemistry, Institute of Genetics & Hospital for Genetic Diseases, Begumpet, 500 016 Hyderahad, India |
| Abstract: | Poly A RNA status and itsin vitro translation in a rabbit reticulolysate cell free system, were assessed in the skeletal muscle of young boys with Duchenne muscular dystrophy. Compared to normals there is a significant 48% increase in poly A RNA content of dystrophic muscle and its translatability was increased by 56% based on35S methionine incorporation into total protein systhesised. Immunoprecipitation of the translated products with monospecific antibodies showed that there is a 2.6 fold and a 2 fold increase in m and μ calpains respectively. This underlines the importance of both synthetic and degenerative activities in the early pathology in DMD muscle. |
| Keywords: | Calpain DMD in vitro translation poly A RNA Protein synthesis Skeletal muscle |
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