An efficient method of Yarrowia lipolytica immobilization using oil- and emulsion-modified bacterial cellulose carriers
Institution:
1. Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Piastów 45, 70-311 Szczecin, Poland;2. Nanomaterials Physicochemistry Department, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Szczecin, Piastów 45, 70-311 Szczecin, Poland;3. Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, Borowska 211A, 50-556 Wroc?aw, Poland;4. Laboratory of Microbiology, PORT Polish Center for Technology Development, Stab?owicka 147, 54-066 Wroc?aw, Poland
Abstract:
BackgroundYarrowia lipolytica is a nonconventional, dimorphic yeast with multiple biotechnological applications. Considering the size of Y. lipolytica cells and a plethora of its morphological forms (spherical cells or hyphae and pseudohyphae), it is highly difficult to select a suitable carrier for this useful microorganism. Bacterial cellulose (BC) is currently considered one of the most promising immobilization carriers. In the current study, the usefulness of oil- and emulsion-modified BCs as a carrier for Y. lipolytica immobilization was investigated. Static and agitated cultures were conducted in media supplemented with oil or emulsion to improve carrier porosity.ResultsIt was found that the application of oil- and emulsion-modified BCs correlated with significantly higher efficiency of Y. lipolytica immobilization and hence higher yield than the yield achieved with an unmodified carrier. Increased efficiency of immobilization correlated with BC porosity-related parameters, which, in turn, depended on the size of oil droplets introduced into the culture medium. Moreover, changes in porosity-related parameters caused by the addition of oil or emulsion to the medium were observed when the cultures were conducted only under static conditions and not under agitated conditions.ConclusionThe application of oil- and emulsion-modified BCs as carriers significantly increased the efficiency of Y. lipolytica immobilization as compared to unmodified BC. The addition of oil or emulsion to the culture medium can be a simple but effective method to modify the porosity of BC-based carriers.How to cite: ?ywicka A, Wenelska K, Junka A, et al. An efficient method of Yarrowia lipolytica immobilization using oil- and emulsion-modified bacterial cellulose carrier. Electron J Biotechnol 2019;41. https://doi.org/10.1016/j.ejbt.2019.06.004.
